Txoj kev npaj rau kev kuaj qhov muag thiab electron microscopy
Kev npaj microscopic feem ntau suav nrog plaub pawg: txoj kev faib, tag nrho mount txoj kev, smear method thiab tableting method.
① Slicing txoj kev. Lub thickness ntawm lub tshuab ntsuas qhov muag yog nyob nruab nrab ntawm 2 thiab 25 microns. Feem ntau, ib daim thickness ntawm li 10 microns rau tsiaj thiab cov ntaub ntawv cog yog tsim nyog. Sectioning txoj kev sib txawv nyob ntawm qhov nruab nrab embedding. Feem ntau siv yog paraffin sectioning method, paj rwb colloid sectioning method, khov sectioning method, thiab ethylene glycol methacrylate method (hu ua GMA method). Cov txheej txheem paraffin sectioning muaj xws li cov kauj ruam xws li fixation, embedding, sectioning, staining, dehydration and mounting. Qhov tseem ceeb yog embed cov khoom lom nyob rau hauv paraffin, siv paraffin raws li kev txhawb nqa, thiab txiav cov khoom lom neeg rau hauv cov quav ciab rau hauv qhov zoo tshaj plaws nyias nyias. Cov txheej txheem kev ua haujlwm yog: kho → dej ntxuav → cawv lub cev qhuav dej ib kauj ruam los ntawm qhov tsis tshua muaj siab mus rau siab concentration → xylene pob tshab → wax dipping → embedding → sectioning → patch → xylene dewaxing → step by step alcohol treatment from high concentration to low concentration. Thaum kawg, nws hloov mus rau dej → dyeing → stepwise dehydration los ntawm qis concentration mus rau siab concentration cawv → xylene pob tshab → resin kua nplaum sealing. Cov kauj ruam yooj yim yog tib yam thoob plaws txhua txoj kev tsim khoom.
②Integral sealing txoj kev. Kev npaj tag nrho-mount rau tib lub hlwb, cov kab mob me me, lossis cov kab mob sib kis. Txoj kev no tseem xav tau ntau yam kev kho, staining, lub cev qhuav dej, pob tshab thiab mounting. Txoj kev no yog siv los npaj paramecia thiab kab qhov ncauj.
③ Smear txoj kev. Ib txoj kev npaj uas yooj yim dispersed biological specimens kis tau rau ntawm ib lub iav swb. Ntshav smear yog ib qho piv txwv.
④Tablet nias txoj kev. Muab cov ntaub so ntswg los yog cov ntaub so ntswg uas yooj yim dispersed tom qab ua tiav, xws li cov tsiaj spermatocytes thiab cov hauv paus tip hlwb, ntawm ib lub iav swb, ntxiv ib lub hau npog, thiab tsoo cov ntaub so ntswg nrog quab yuam kom cov hlwb los yog cov hlwb hauv cov qauv. kis mus rau hauv ib txheej ntawm cov txheej txheem ntau lawm. Txoj kev nias yog feem ntau siv los soj ntsuam cov chromosomes, feem ntau stained nrog magenta acetate, lichen liab thiab carbolic fuchsin.
