Kev ceev faj thiab kev saib xyuas ntawm fluorescence microscope
(1) Ua haujlwm nruj raws li cov cai ntawm fluorescence microscope Hoobkas phau ntawv, tsis txhob hloov cov txheej txheem ntawm kev xav.
(2) Nws yuav tsum tau kuaj xyuas hauv chav tsaus, tig rau lub zog hluav taws xob ntawm lub tshuab hluav taws xob siab tom qab nkag mus rau hauv chav tsaus, tig rau lub teeb pom kev kub siab mercury tom qab tig rau 3 ~ 5 nag, thiab tom qab ntawd tig rau excitation siab siab mercury teeb, thiab nres qhov excitation thaum koj pom tias lub siab kub siab mercury teeb yog ci kiag li, ces pib soj ntsuam cov qauv.
(3) Tiv thaiv UV puas rau ob lub qhov muag, muab tso rau ntawm qhov screen tiv thaiv ua ntej ua qhov kev sim.
(4) Txheeb xyuas lub sijhawm txhua lub sijhawm mus rau 1h yog qhov tsim nyog, ntau dua 90min, lub zog siab mercury teeb luminous siv maj mam txo qis, fluorescence tsis muaj zog, cov qauv zoo siab 15min, fluorescence kuj tseem tsis muaj zog.
(5) fluorescence microscope excitation ntaus ntawv thiab high-pressure mercury teeb lub neej yog txwv, cov qauv yuav tsum yog centralized soj ntsuam, txuag lub sij hawm; mercury teeb yuav tsum zam ntau qhov qhib, * zoo kom tsis txhob hloov hauv ib nrab teev.
(6) Saib xyuas cov qauv tam sim tom qab staining, vim tias lub sijhawm cia ntev dhau lawm, cov fluorescence yuav maj mam tawg. Muaj peev xwm stained cov ntaub qhwv rau hauv daim ntawv dub, khaws cia rau hauv hnab yas polyethylene, 4 ~ C cia, tuaj yeem ncua lub sijhawm tawg fluorescence.
(7) Cov qauv txiav txim ntawm fluorescence brightness feem ntau yog plaub theem, piv txwv li 'a' tsis muaj lossis pom tsis muaj zog autofluorescence; '+' tsuas pom fluorescence; '++' pom ci fluorescence. fluorescence; '+++' pom dazzling fluorescence.
Kev ceev faj thiab kev saib xyuas ntawm fluorescence microscope:
1. Lub lens ntxuav cov plua plav daig ntawm lub lens yuav tsum yog cov txhuam hniav mos mos, maj mam txhuam tawm, nyob rau hauv qhov chaw ntawm cov ntiv tes thiab cov roj stains yuav tsum mos hauv cov net ntawm degreasing paj rwb, gauze los yog daim iav daim ntawv dipped nyob rau hauv anhydrous ethanol (los yog methanol) maj mam so, nyob rau saum npoo ntawm lub hom phiaj lens yuav siv tau los so cov roj stains nrog roj av.
2. Xim ib feem, yas ib feem ntawm kev ntxuav ntawm cov xim pleev xim, ib feem ntawm cov xim ntawm cov xim tsis muaj xim tshem tawm, tsis txhob siv cov kuab tshuaj organic.
