Kev ceev faj thiab kev saib xyuas ntawm confocal microscope
Muaj ntau hom qauv uas tuaj yeem ntsuas los ntawm confocal microscopy. Xws li cov khoom siv roj ntsha, cov ntaub so ntswg (section), cell (subcellular) qauv, thiab lwm yam. Lub ntsiab qhov chaw ntawm fluorescence nyob rau hauv cov qauv yog raws li nram no: autofluorescence, fluorescent staining, ** fluorescence, fluorescent protein, induced fluorescence thiab enzymatically tsim fluorescence, thiab lwm yam. Cov excitation thiab emission wavelengths ntawm feem ntau ntawm cov fluoresceins tuaj yeem pom nyob rau hauv cov ntaub ntawv dyeing cov tsev qiv ntawv ntawm cov cuab yeej software.
Confocal Microscope Instrument Parameters
Laser:
Ultraviolet laser: 405nm, lub zog> 30 mW;
Ar ion laser: 458/476/488/496/514nm, power>50 mW;
Dawb laser: pulsed laser, 470-670nm, hwj chim Ntau dua los yog sib npaug rau 1mW, kauj ruam 1nm, siv tau 8 laser kab tib lub sij hawm, mem tes zaus 80MHz-10MHz adjustable.
Lub Hom Phiaj Lens:
Npaj apochromatic confocal mob siab rau fluorescence lub hom phiaj.
Ntes:
Muaj ob lub PMT detectors thiab ob lub HyD ntes, ib qho yog HyD SMD detector nrog ib leeg-molecule detection muaj nuj nqi.
Duab nrhiav thiab ua:
LAS X software (saib ua ntej, sib cais xim xim, mosaic, ntau lub ntsiab lus tau txais, kev txheeb xyuas cov ntaub ntawv), SymPho Sijhawm 64 (lub neej duab, cov ntaub ntawv haum), Huygens Professional (duab deconvolution processing, visualization thiab ** tsom xam, thiab lwm yam) .
Live Cell Workstation:
Kev tswj kub thiab CO2 premixing.
Kev ceev faj thiab kev saib xyuas ntawm confocal microscope
Khaws kom deb ntawm qhov chaw hluav taws xob hluav taws xob nyob ib puag ncig lub ntsuas;
Tsis muaj kev vibration nyob rau hauv ib puag ncig, tsis muaj zog huab cua cuam tshuam;
Muaj cov txheej txheem shading nyob rau hauv chav tsev kom ntseeg tau tias cov qauv fluorescent yuav tsis bleached los ntawm lub teeb sab nraud;
Ib puag ncig huv;
Tswj kev ua haujlwm kub ntawm 5-25 degree .
