Polarizing microscope siv thiab ceev faj
Polarizing microscope yog ib hom kev tsom xam siv los kawm cov ntaub ntawv pob tshab thiab opaque anisotropic. Nws muaj cov ntawv thov tseem ceeb hauv geology thiab lwm yam kev tshawb fawb thiab engineering majors. Txhua yam khoom uas muaj birefringence tuaj yeem pom meej meej nyob rau hauv lub tshuab ntsuas polarizing. Tau kawg, cov tshuaj no kuj tuaj yeem pom los ntawm staining, tab sis qee yam ntawm lawv tsis muaj, thiab yuav tsum siv lub tshuab ntsuas polarizing. Lub tshuab tsom iav polarizing yog ib qho cuab yeej tseem ceeb rau kev tshawb fawb thiab kev txheeb xyuas cov tshuaj birefringent los ntawm kev siv cov yam ntxwv ntawm lub teeb. Nws tuaj yeem siv rau kev soj ntsuam lub teeb pom kev zoo ib yam, kev soj ntsuam lub teeb orthogonal polarized, thiab kev soj ntsuam lub teeb conoscopic.
siv
Polarizing microscope yog ib qho cuab yeej tseem ceeb rau kev kawm cov khoom siv kho qhov muag ntawm cov muaju, thiab nws kuj yog lub hauv paus ntawm lwm txoj kev tshawb fawb siv lead ua (roj immersion method, Freund's method, etc.).
Polarizing microscope yog ib qho cuab yeej tseem ceeb rau kev tshawb fawb thiab kev txheeb xyuas cov tshuaj birefringent los ntawm kev siv cov yam ntxwv ntawm lub teeb. Nws tuaj yeem siv rau kev soj ntsuam lub teeb pom kev zoo ib yam, kev soj ntsuam lub teeb orthogonal polarized, thiab kev soj ntsuam lub teeb conoscopic. Ib txoj hauv kev hloov lub teeb zoo tib yam rau hauv lub teeb polarized rau kev kuaj xyuas lub tshuab ntsuas kom paub seb cov khoom puas yog ib qho refraction (isotropic) lossis birefringence (anisotropy).
Birefringence yog ib qho tseem ceeb ntawm cov muaju. Yog li ntawd, polarizing microscopes tau siv dav hauv cov ntxhia, tshuaj thiab lwm yam. Nyob rau hauv tib neeg thiab zoology, polarized light microscopy feem ntau yog siv los txheeb xyuas cov pob txha, cov hniav, cov roj cholesterol, cov hlab ntsha, cov qog hlwb, cov leeg nqaij thiab cov plaub hau. Hnub no, kuv yuav qhia txog daim ntawv thov teb ntawm polarizing microscopes.
1. Biological teb:
Hauv cov kab mob uas muaj sia nyob, cov qauv fibrin sib txawv qhia tau hais tias anisotropy, thiab kev siv lub teeb pom kev zoo polarized tau txais cov ntsiab lus ntawm cov txheej txheem molecular hauv cov fibers. Xws li collagen, spinning txhob lo lo ntxhuav thaum lub sij hawm faib cell, thiab lwm yam.
2. Kev txheeb xyuas ntawm ntau yam khoom siv roj ntsha thiab tsis muaj roj ntsha: xws li cov khoom siv hmoov txhuv nplej siab, kev txheeb xyuas cov tshuaj muaj pes tsawg leeg, fiber ntau, kua siv lead ua kua, DNA siv lead ua, thiab lwm yam.
3. Kev tshuaj xyuas kev kho mob: xws li pob zeb, uric acid crystal detection, mob caj dab, thiab lwm yam.
4. Geological thiab mineral tsom xam:
Ntxiv nrog rau kev siv tshuaj biomedical, polarized lub teeb microscopes kuj tseem siv tau rau kev tshawb pom lub teeb pom kev ntawm ntau yam minerals thiab crystals, thiab siv dav hauv roj av, mining thiab semiconductor industries. LED teeb pom kev zoo thiab cov ntxaij lim dej tshwj xeeb tuaj yeem siv rau hauv kev tswj kom zoo thiab kev tsom xam kev lag luam.
Txhawm rau kom ntseeg tau tias kev pabcuam lub neej thiab kev ntseeg siab ntawm qhov system, ua tib zoo saib rau cov khoom hauv qab no:
1. Lub chaw soj nstuam yuav tsum muaj peb yam kev tiv thaiv: kev poob siab (tawm ntawm qhov chaw ntawm kev poob siab), kev tiv thaiv dej noo (siv cua txias, tshuab ziab khaub ncaws), plua plav pov thawj (pem hauv ntej); Fais fab mov: 220V ntxiv rau -10 feem pua, 50HZ kub: 0-40 degrees.
2. Thaum kho kom pom tseeb, ceev faj tsis txhob cia lub hom phiaj lens kov tus qauv, thiaj li tsis txhob khawb lub hom phiaj lens.
3. Tsis txhob hloov lub hom phiaj lens thaum qhov nruab nrab ntawm lub qhov puag ncig ntawm lub theem spacer nyob ze rau qhov chaw ntawm lub hom phiaj lens, thiaj li tsis mus khawb lub hom phiaj lens.
4. Tsis txhob kho qhov ci ntsa iab tam sim ntawd, thiab tsis txhob ci dhau, uas yuav cuam tshuam rau lub neej kev pab cuam ntawm lub teeb thiab tseem ua rau qhov muag tsis pom kev.
5. Rau tag nrho (function) switching, qhov kev txiav txim yuav tsum yog lub teeb thiab nyob rau hauv qhov chaw.
6. Tshem tawm qhov ci kom tsawg.
7. Cov kws tshaj lij yuav tsum tsis txhob kho lub teeb pom kev zoo (filament txoj hauj lwm teeb), thiaj li tsis cuam tshuam rau cov duab zoo.
8. Ua tib zoo saib xyuas qhov kub thiab txias thaum hloov lub teeb halogen kom tsis txhob kub hnyiab; ceev faj tsis txhob kov lub iav lub cev ntawm lub teeb halogen ncaj qha nrog koj txhais tes.
9. Thaum lub tshuab tsis siv, kho lub hom phiaj lens mus rau lub xeev qis tshaj los ntawm kev tsom xam.
10. Thaum lub tshuab raug kaw thiab tsis siv, tsis txhob npog cov plua plav npog tam sim ntawd, thiab npog nws tom qab nws txias. Ua tib zoo saib xyuas kev tiv thaiv hluav taws.
11. Cov khoom siv kho qhov muag uas tsis nquag siv tau muab tso rau hauv lub tais kom qhuav.
12. Cov uas tsis yog kws tshaj lij yuav tsum tsis txhob sim so lub hom phiaj lens thiab lwm yam khoom siv kho qhov muag. Cov eyepiece tuaj yeem so nrog paj rwb swab dipped nyob rau hauv ib qho piv txwv 1: 1 (tsis muaj cawv: ether) tov kua, thiab ces so. Tsis txhob siv lwm cov kua dej kom tsis txhob muaj kev puas tsuaj rau lub qhov muag.
