Txoj kev ua microscope slides
Muaj peb txoj hauv kev los ua microscope slides:
1. Smear txoj kev
Smear txoj kev yog ib txoj kev npaj uas cov khoom yog kis tusyees ntawm tus swb.
Cov khoom smear muaj xws li cov kab mob unicellular, me me algae, ntshav, kab mob kab mob, cov ntaub so ntswg ntawm cov nroj tsuag thiab tsiaj txhu, phev zes, anthers, thiab lwm yam.
Nco ntsoov yuav tsum tau them thaum smearing:
(1) Lub swb yuav tsum huv.
(2) Lub swb yuav tsum tuav ncaj nraim.
3) Cov txheej yuav tsum yog uniform. Siv cov tee dej ntawm cov kua nplaum rau sab xis ntawm qhov nruab nrab ntawm cov swb thiab kis tau zoo nrog cov hniav ntawm rab riam los yog ib tug txhuam hniav, thiab lwm yam.
4) Cov txheej yuav tsum nyias. Siv lwm tus swb ua tus thawb, maj mam thawb los ntawm sab xis mus rau sab laug raws qhov chaw ntawm cov swb nrog kev poob ntawm daim ntawv thov kev daws teeb meem (lub kaum sab xis ntawm ob qhov swb yuav tsum yog 30 degree txog 45 degree), thiab siv cov txheej nyias nyias.
5) Kev kho. Yog xav tau kev kho, siv tshuaj fixative los yog desiccation (bacterial) fixation.
6) Staining. Siv methylene xiav rau cov kab mob thiab Rachel cov stain rau ntshav. Cov tshuaj staining yuav tsum npog tag nrho cov coated nto.
7) Yaug. Absorb on ntawv absorbent los yog ci qhuav.
8) Kaw zaj duab xis. Rau kev khaws cia ntev, siv Canadian ntoo zaj duab xis.
2. Txoj kev nias zaj duab xis
Txoj kev ua yeeb yaj kiab siab yog cov khoom siv lom neeg muab tso rau nruab nrab ntawm daim ntawv swb thiab npog, siv ib qho kev siab, cov ntaub so ntswg yuav raug nias sib nrug ib txoj kev npaj.
(1) nqa cov khoom. Kev soj ntsuam ntawm cell division, cell division yuav tsum xaiv exuberant, tshiab cov ntaub so ntswg cell raws li cov ntaub ntawv. Xws li cov lus qhia hauv paus, qia meristematic cov ntaub so ntswg, pob txha hlwb hlwb, anthers (pollen niam hlwb). Sperm zes (spermatogonia) thiab lwm yam.
(2) Kev kho. Kev kho cov khoom siv tuaj yeem txiav txim siab raws li qhov xav tau, tam sim ntawd tom qab kev soj ntsuam cov khoom siv siab, tsis tuaj yeem ua rau kev kho mob sib cais (thiab staining synchronous); noj cov khoom tsis yog tam sim ntawd tom qab kev tshuaj xyuas, cov khoom tuaj yeem kho nrog lub fixative (feem ntau nrog cawv acetate fixative). Tsau 2 ~ 24 teev (nyob ntawm cov khoom siv) tom qab ntxuav nrog 95% ethanol, khaws cia hauv 70% ethanol, standby.
(3) Kev tsis sib haum xeeb. Cov hlwb tsis yooj yim rau kev tawg ntawm cov khoom siv nrog hydrolysis sib cais kev daws teeb meem (xws li 1N HCl lossis hydrochloric acid ib qho dej cawv) kev kho mob, kev kho mob dav dav 6 ~ 20min, tom qab dissociation thiab yaug nrog dej ua ntej staining.
(4) Staining. Muaj ntau hom stains. Kev soj ntsuam ntawm chromosomes feem ntau yog siv los stain nrog magenta acetate staining tov.
(5) Zaj duab xis nias. Muab cov khoom tso rau ntawm ib lub swb, ntxiv ib tee dej los yog staining tov, npog lub npog thiab maj mam nias tus swb nrog koj tus ntiv tes xoo.
(6) Kev soj ntsuam. Tom qab nias cov yeeb yaj kiab, nws tuaj yeem pom nyob rau hauv lub tshuab tsom.
