Cov khoom tseem ceeb thiab kev ua haujlwm ntawm laser confocal microscope
1. Illuminating pinhole
Kev Ua Haujlwm: Lub laser hla dhau lub teeb pom kev zoo los tsim qhov chaw teeb pom kev zoo. Cov ntsiab lus teeb pom kev zoo muaj qhov zoo xws li lub teeb pom kev zoo, qhov sib txawv me me, qhov ci ci siab, siab spatial thiab temporal coherence, thiab dav hlau polarization excitation. Thiab tsim ib tug confocal ntaus ntawv nrog lub detector pinhole thiab focal dav hlau.
2. Beam splitter
Muaj nuj nqi: cais cov qauv excitation fluorescence los ntawm lwm lub teeb uas tsis yog teeb liab.
3. Lub hom phiaj lens
4.Focal dav hlau
Kev Ua Haujlwm: Lub laser point lub teeb ci ci qhov khoom thiab tsom nws ntawm lub dav hlau focal, txhawb nqa cov fluorescently sau cov qauv kom tawm fluorescence los ua ib qho chaw focal. Lub teeb pom kev zoo yog ua tiav los ntawm ntau cov khoom siv xws li lub hom phiaj lens thiab beam splitter, thiab tsom mus rau lub teeb pom kev pinhole thiab lub ntsuas ntsuas pinhole feem. Qhov no yog qhov uas lub ntsiab lus ntawm confocal los ntawm.
5. Ntes pinhole
Function: Ua raws li cov lim dej spatial. Ua kom muaj kev cuam tshuam ntawm lub dav hlau uas tsis yog tsom mus rau lub teeb tawg thiab tawg tawg tawm sab nraud ntawm qhov chaw tsis tsom rau ntawm lub dav hlau tsom kom ntseeg tau tias tag nrho cov teeb liab fluorescence tau txais los ntawm tus ntes pinhole los ntawm qhov chaw ua kom pom tseeb ntawm qhov chaw coj mus kuaj, yog li qhov diffraction tsom qhov chaw ntawm cov qauv thiab kuaj pom Lub pinhole imaging chaw muaj cov ntaub ntawv tib yam (ob lub ntsiab lus conjugate).
6. Photomultiplier tube (detector)
Muaj nuj nqi: Tau txais lub teeb liab hla lub pinhole, hloov mus rau hauv lub teeb liab hluav taws xob thiab xa mus rau lub khoos phis tawj, kom pom cov duab meej ntawm tag nrho lub dav hlau focal tshwm ntawm qhov screen.
7. Lasers: Txoj kev loj hlob ntawm confocal microscopy technology yog inseparable los ntawm kev loj hlob sai ntawm lasers. Peb tuaj yeem xaiv cov lasers sib txawv raws li kev tshawb fawb xav tau. Xws li ArUV (351.364nm), HeCd (442nm), AR (457, 488, 514nm), ArKr (488, 568, 647nm) Kr (568nm), HeNe (543nm), HeNe (633nm), thiab lwm yam.
8. Ntau cov fluorescence channels: Nws muaj ntau yam fluorescence raws kom ua tiav ntau cov ntawv sau ntawm cov qauv tib lub sijhawm.
