Cov Cheebtsam tseem ceeb thiab cov haujlwm ntawm Laser Infocal tsom iav
1. Lub teeb liab pinhole
Muaj nuj nqi: Ua rau lub laser dhau los ntawm cov isturmination pinhole los tsim cov kev coj ua zoo xws li muaj zog, siab dua thiab cov phiaj xwm kev ua kom zoo nkauj. Thiab nws tsim cov cuab yeej ua tiav nrog lub ntes pinhole thiab focal dav hlau.
2. Beam Splitter
Muaj nuj nqi: cais cov qauv ua kom tsaug zog fluorescence los ntawm lwm yam tsis muaj teeb liab.
3. Lub hom phiaj Lens
4. Focal dav hlau
Muaj nuj nqi: Lub teeb ci ntsa iab taw qhia rau cov khoom ntawm lub dav hlau hauv qab, txaus siab rau cov fluorescented coj los tso cov fluorescence thiab tsim lub focal chaw. Lub teeb pom kev yog ua tiav los ntawm cov khoom siv hluav taws xob xws li lub hom phiaj stip thiab tsom ntawm ob qhov chaw: Lub Illumination pinhole thiab cov ntes pephole. Lub ntsiab lus ntawm kev ua tiav los ntawm qhov no.
5. Ntes Pinhole
Muaj nuj nqi: ua si raws li lub lim spatial. * Txhawm rau kom ua kom ntau tshaj plaws ntawm kev ntawm cov teeb meem tsis tsom iav tawg, kom paub meej tias txhua qhov chaw f f f f f f f f f f f f f f f f f f f f f focal ntawm cov qauv ntsuas. Therefore, the diffraction focused spot on the sample and the detector pinhole imaging spot contain the same information (two-point conjugate).
6. Photomultiplip Thia (Ntes)
Muaj nuj nqi: Tau txais lub teeb pom kev los ntawm pinholes, hloov lawv mus rau lub khoos phis tawm, ua rau lub computer ntshiab ntawm lub vijtsam puv nkaus.
7. Peb tuaj yeem xaiv lasers sib txawv raws li peb cov kev tshawb fawb xav tau. Nyob rau hauv kev ua raws li Aruv (351.364nm), HECD (442NM), AR (488,5147nM), HENE (543NM), HEE (633NM) tos.
8. Ntau ntau fluorescence raws: Muaj ntau cov nqe lus fluorescence kom ua tiav ntau daim cim ntawm cov qauv sib dhos.
