Laser Confocal Scanning Microscope
Laser confocal scanning microscope (laser confocal scanning microscope), siv laser li scanning lub teeb qhov chaw, ceev scanning thiab imaging point los ntawm taw tes, kab los ntawm kab, thiab nto los ntawm kab, lub scanning laser thiab fluorescence sau qhia lub hom phiaj lens, thiab lub hom phiaj lens. scans lub focal point ntawm lub teeb laser, uas kuj yog instantaneous imaging point ntawm teeb meem. Vim hais tias lub wavelength ntawm lub laser beam yog luv luv thiab lub beam yog heev nyias, lub confocal laser scanning microscope muaj ib tug siab daws teeb meem, uas yog hais txog 3 npaug ntawm qhov zoo tib yam optical microscope. Tom qab lub kaw lus tsom ib zaug, qhov kev tshuaj ntsuam tsuas yog txwv rau ib lub dav hlau ntawm cov qauv. Thaum lub tsom qhov tob sib txawv, cov duab ntawm qhov sib txawv qhov tob ntawm cov qauv tuaj yeem tau txais. Cov ntaub ntawv duab no khaws cia rau hauv lub computer. Los ntawm kev txheeb xyuas lub computer thiab simulation, peb-seem qauv ntawm cov qauv ntawm tes tuaj yeem tshwm sim.
Vim li cas koj thiaj xav tau confocal microscope?
1. Tom qab kev siv zog thiab kev txhim kho ntawm peb cov thawj coj zoo, lub tshuab kho qhov muag tau mus txog qhov zoo tshaj plaws. Qhov tseeb, lub tshuab microscopes zoo tib yam tuaj yeem muab peb cov duab zoo nkauj microscopic yooj yim thiab sai. Txawm li cas los xij, ib qho kev tshwm sim uas coj kev hloov pauv hloov tshiab rau lub ntiaj teb no yuav luag zoo meej lub tshuab tsom iav tau tshwm sim, uas yog qhov tsim ntawm "laser scanning confocal microscope". Qhov tshwj xeeb ntawm hom tshiab ntawm lub tshuab ntsuas ntsuas no yog tias nws siv lub tshuab kho qhov muag uas tsuas yog rho tawm cov duab cov ntaub ntawv ntawm qhov chaw uas qhov kev ua kom pom tseeb, thiab rov kho cov ntaub ntawv tau txais hauv daim duab nco thaum hloov qhov ua kom pom tseeb, kom ua tiav cov ntaub ntawv 3D tuaj yeem ua tau. tau. Ib tug stark duab ntawm kev txawj ntse. Nrog rau txoj kev no, nws tuaj yeem yooj yim tau txais cov ntaub ntawv hais txog lub ntsej muag zoo uas tsis tuaj yeem lees paub nrog lub tshuab tsom iav ib txwm. Tsis tas li ntawd, rau qhov zoo tib yam optical microscopes, "kev daws teeb meem" thiab "qhov tob ntawm kev tsom mus rau" yog qhov tsis sib xws, tshwj xeeb tshaj yog nyob rau hauv qhov siab, qhov kev tsis sib haum xeeb no tseem ceeb dua, tab sis hais txog confocal microscopes, qhov teeb meem no yooj yim daws tau.
2. Qhov zoo ntawm confocal optical system
Schematic daim duab ntawm laser confocal microscope
Lub confocal optical system ua cov ntsiab lus illumination ntawm tus qauv, thiab lub teeb pom kev zoo kuj tau txais los ntawm tus taw tes receptor. Thaum cov qauv muab tso rau hauv txoj hauj lwm ua kom pom tseeb, yuav luag tag nrho cov teeb pom kev zoo tuaj yeem ncav cuag photoreceptor, thiab thaum tus qauv tsis ua kom pom tseeb, lub teeb pom kev tsis tuaj yeem ncav cuag photoreceptor. Uas yog hais tias, nyob rau hauv confocal optical system, tsuas yog cov duab uas coincides nrog lub focal point yuav tso zis, thiab lub teeb me ntsis thiab tsis muaj txiaj ntsig tawg yuav raug shielded.
3. Vim li cas thiaj siv laser?
Nyob rau hauv lub confocal optical system, tus qauv yog illuminated ntawm ib tug taw tes, thiab lub reflected lub teeb kuj tau txais los ntawm ib tug point photoreceptor. Yog li ntawd, ib tug point light qhov chaw yuav tsum tau. Lasers yog qhov chaw teeb pom kev zoo heev. Feem ntau, cov teeb pom kev laser yog siv los ua lub teeb ci rau confocal microscopes. Tsis tas li ntawd, cov yam ntxwv ntawm monochromaticity, directionality thiab zoo heev beam duab ntawm laser kuj tseem ceeb vim li cas rau nws dav saws.
4. Kev soj ntsuam tiag tiag raws li kev soj ntsuam ceev ceev tuaj yeem ua tau
Rau laser scanning, kab rov tav kev taw qhia adopts Acoustic Optical Deflector (AO lub ntsiab), thiab ntsug kev taw qhia txais lub servo electronically tswj beam scanning daim iav (Servo Galvano-mirror). Txij li thaum lub acousto-optical deflection chav tsev tsis muaj ib qho kev co, nws muaj peev xwm ua tau high-speed scanning thiab real-time kev soj ntsuam ntawm lub monitor screen yog ua tau. Qhov kev ntsuas siab ceev no yog ib qho tseem ceeb heev uas cuam tshuam ncaj qha rau kev ceev ntawm kev tsom thiab qhov chaw retrieval.
