Cov yam tseem ceeb uas yuav tsum tau xav txog thaum yuav lub tshuab tsom.
Upright los yog upside down?
Tau kawg, nyob ntawm daim ntawv thov npaj thiab cov qauv uas koj xav tau soj ntsuam, nws yuav pab koj txiav txim siab tam sim ntawd seb koj xav tau lub tshuab hluav taws xob ncaj qha lossis lub tshuab ntsuas inverted, qhov txawv yog qhov optics nyob rau saum toj lossis hauv qab cov qauv. "Tab sis lwm yam uas yuav tsum xav txog tsis yog tsuas yog tus qauv xwb, tab sis kuj yog lub nkoj uas koj tab tom saib," hais tias Olympus tus lwm yam khoom lag luam Lauren Alvarenga. Rau kev kuaj xyuas microscopic ntawm cov qauv hauv cov tais diav los yog cov tais yas, koj yuav xav tau lub tshuab ntsuas qhov muag nrog lub ntsej muag uas muaj qhov ntev ua haujlwm kom haum rau lub thickness ntawm lub tais.
kho qhov muag system
Kev daws lub zog yog lub peev xwm los saib cov qauv qauv thiab qhov nrawm ntawm cov duab tuaj yeem daws tau - lwm qhov tseem ceeb uas yuav tsum tau xav txog.
"Lub hom phiaj yog qhov tseem ceeb hauv kev kho qhov muag," hais txog Jeff McGinn, tus thawj tswj hwm ntawm McCrone Microscopy thiab Accessories, kev faib khoom ntawm McCrone Group. "Qhov siab dua tus lej aperture (NA) ntawm lub hom phiaj, nws qhov kev daws teeb meem zoo dua. Peb ntsuas qib ntawm cov ntsiab lus uas tus neeg siv yuav tsum tau pom thiab pob nyiaj siv, los pab cov neeg siv xaiv lub hom phiaj lens nrog qhov tsim nyog tus lej aperture (NA). " Ntxiv nrog rau cov lej aperture (NA), nws kuj tseem yuav tsum tau soj ntsuam cov qib ntawm kev kho ntawm kheej kheej aberration thiab chromatic aberration ntawm lub tsom iav tsom iav tsom iav.
Hais txog kev siv nyiaj, Alvarenga qhia: "Xaiv lub hom phiaj hauv koj cov peev nyiaj. Qhov zoo ntawm lub hom phiaj yuav cuam tshuam ncaj qha rau cov ntaub ntawv zoo, yog li qhov no yog qhov tseem ceeb heev uas yuav tsum xav txog." Koj tuaj yeem xaiv cov hom phiaj tsim los rau cov tswv yim tshwj xeeb, xws li theem kev sib piv imaging, silicone optical time-lapse imaging. Phase contrast imaging yog feem ntau siv nyob rau hauv ntub chaw soj nstuam, tshwj xeeb tshaj yog cov uas ua cell counts los yog viability assay. Nyob rau tib lub sijhawm, xyuas qhov ua haujlwm nrug ntawm lub hom phiaj lens (uas yog, WD, qhov deb ntawm lub lens thiab cov qauv) los txiav txim seb nws puas phim koj hom qauv.
kuaj pom
Lwm qhov tseem ceeb ntawm txoj kev teeb pom kev hauv lub tshuab hluav taws xob yog lub cuab yeej nrhiav tau rau cov qauv teeb liab.
"Lub koob yees duab thev naus laus zis tab tom txhim kho sai," Olenych tau hais tias, ceeb toom tias qhov kev thov rau CMOS lub koob yees duab chips tau nce nrawm dua piv rau cov cuab yeej siv CCD. CMOS chips feem ntau loj dua qhov loj thiab rhiab dua li CCD chips, nws piav qhia. CMOS chips muaj ntau pixels, uas txhais tau hais tias lawv tuaj yeem ua tiav cov duab zoo dua hauv cheeb tsam me. Photomultiplier tubes (PMTs) kuj yog ib qho kev xaiv hauv cov ntawv thov uas tsis xav tau kev daws teeb meem spatial. PMTs tsis khaws cov nqi thiab tuaj yeem teb rau cov kev hloov pauv hauv lub teeb tawm hauv nanoseconds, yog li lawv tau siv los ntes thiab sau cov xwm txheej ceev heev.
yooj yim ntawm kev siv
Lwm qhov tseem ceeb uas yuav cuam tshuam koj qhov kev txiav txim siab yog qhov yooj yim ntawm kev siv lub tshuab tsom iav. Tus neeg siv-phooj ywg ntawm ib qho cuab yeej yog nyob ntawm qhov software interface. Alvarenga tau hais tias "Olympus software muaj ntau heev nyob rau txhua lub tshuab hauv qab nws cov hom, txo lub sijhawm rov tshawb xyuas cov txheej txheem ntawm lub tshuab tsom iav tshiab thiab cob qhia cov neeg ua haujlwm tshiab," Alvarenga tau hais. Qee cov kws tsim kho tshuab microscope tau qhia ntau qhov kev nkag siab zoo, tswj cov cuab yeej los ntawm kev siv tshuab touchscreen. Piv txwv li, Revolve microscope los ntawm Echo Laboratories yog tswj los ntawm ib qho App nyob rau hauv lub iOS no system, uas muaj ib tug pinch-rau-zoom muaj nuj nqi, thiab kuj yuav ua tau cell suav los ntawm txhaj rau qhov screen. Eugene Cho, CEO thiab tus tsim ntawm Echo Laboratories, piav qhia tias: "Kev yooj yim ntawm kev siv, uas yog qhov kev txhawj xeeb tsawg tshaj plaws rau cov neeg siv, tau dhau los ua qhov tseem ceeb rau cov neeg siv.
