Taw qhia txog qhov zoo thiab qhov tsis zoo ntawm multiphoton laser scanning microscopy
1. Siv lub teeb liab lossis lub teeb infrared los ua kom zoo siab, lub teeb tawg yog me me, thiab tawg ntawm cov khoom me me yog qhov sib npaug ntawm plaub lub zog ntawm lub wavelength.
2. Ntau cov photons tawg los ntawm cov duab hla ntu tuaj yeem sau tau yam tsis xav tau cov pinholes.
3. Lub pinhole tsis tuaj yeem paub qhov txawv txav ntawm cov photons tawm ntawm thaj chaw defocused lossis thaj chaw tsom, thiab cov teeb liab-rau- suab nrov piv ntawm ntau cov duab duab hauv cov txheej sib sib zog nqus yog qhov zoo.
4. Lub teeb ci ultraviolet lossis pom pom siv rau ib leeg-photon excitation yog yooj yim absorbed thiab attenuated los ntawm cov qauv ua ntej lub beam mus txog lub focal dav hlau, thiab yog ib qho nyuaj rau excite sib sib zog nqus txheej.
5. Nyob rau hauv cov nqe lus ntawm kev soj ntsuam ntawm lub tshuab microscope, thawj qhov kev txiav txim siab tsis yog ua kom lub cev muaj zog ntawm nws tus kheej thiab tswj cov dej ntws, ion concentration, oxygen thiab cov as-ham. Nyob rau hauv cov ntaub ntawv ntawm kev soj ntsuam lub teeb, ob qho tib si tshav kub thiab photon zog yuav tsum nyob rau hauv lub irradiation npaum li cas thiab lub teeb lub zog uas lub hlwb yuav tsis puas.
6. Multiphoton microscopy muaj ntau yam zoo. Xws li kev daws teeb meem peb-dimensional, qhov tob nkag mus, kev ua haujlwm tawg, lub teeb tom qab, teeb liab-rau-suab nrov piv, tswj, thiab lwm yam, txhua tus muaj cov yam ntxwv uas yav dhau los laser microscopes tsis muaj lossis tsis tuaj yeem sib piv nrog.
Disadvantages ntawm multiphoton laser scanning microscopy:
1. Nws tuaj yeem tsuas yog duab fluorescence.
2. Yog tias cov qauv muaj chromophores uas nqus cov teeb pom kev zoo, cov qauv yuav raug thermally puas.
3. Qhov kev daws teeb meem yog txo me ntsis. Txawm hais tias nws tuaj yeem txhim kho los ntawm kev siv confocal qhov tib lub sijhawm, muaj teeb liab poob.
4. Txwv los ntawm kim ultrafast lasers, tus nqi ntawm multiphoton scanning microscopes yog tus nqi siab.
