Taw qhia ntawm cov khoom tseem ceeb thiab kev ua haujlwm ntawm laser confocal microscope
1. Teeb pom kev zoo pinhole Muaj nuj nqi: Ua kom lub laser hla dhau lub teeb pom kev los tsim lub teeb pom kev zoo, uas muaj qhov tshwj xeeb zoo ntawm kev muaj zog directivity, me me divergence, siab brightness, siab spatial thiab temporal coherence thiab dav hlau polarization excitation. Thiab tsim ib tug confocal ntaus ntawv nrog tus pinhole thiab focal dav hlau ntawm lub detector.
2. Beam splitter Function: cais qhov zoo siab fluorescence ntawm tus qauv los ntawm lwm yam tsis muaj teeb liab teeb.
3. Lub hom phiaj lens
4. Focal plane Function: Lub laser point light source irradiates cov khoom kom pom tseeb ntawm lub dav hlau focal, thiab excites cov fluorescent sau cov qauv kom emit fluorescence, tsim ib tug focal chaw. Qhov chaw yog ua tiav los ntawm ntau cov khoom siv, xws li lub hom phiaj lens thiab beam splitter, thiab tsom mus rau lub teeb pom kev zoo pinhole thiab tus ntes pinhole feem. Lub ntsiab lus ntawm confocal los ntawm qhov no.
5. Ntes pinhole muaj nuj nqi: Nws ua raws li lub spatial lim. * Thaiv lub teeb tawg los ntawm lub dav hlau tsis tsom thiab lub teeb tawg los ntawm qhov chaw tsis pom kev ntawm lub dav hlau tsom mus rau qhov siab tshaj plaws, txhawm rau xyuas kom meej tias tag nrho cov teeb liab fluorescence tau txais los ntawm tus ntes pinhole los ntawm qhov chaw tsom ntawm cov qauv qhov chaw, yog li qhov diffraction tsom qhov chaw ntawm tus qauv thiab qhov chaw imaging ntawm lub detector pinhole muaj tib cov ntaub ntawv (ob lub ntsiab lus conjugate).
6. Photomultiplier tube (detector) Muaj nuj nqi: Tau txais lub teeb liab optical hla dhau lub pinhole, hloov mus rau hauv hluav taws xob teeb liab thiab xa mus rau lub computer, thiab cov duab meej ntawm tag nrho cov focal dav hlau yuav tshwm sim ntawm qhov screen.
7. Laser: txoj kev loj hlob ntawm confocal microscope technology yog inseparable los ntawm kev loj hlob sai ntawm lasers. Peb tuaj yeem xaiv cov lasers sib txawv raws li kev tshawb fawb xav tau. Xws li ARUV (351.364 nm), HECD (442 nm), AR (457,488,514 nm), ARKR (488,568,647 nm) KR (568 nm), Hene (543 nm), Hene (633 nm) thiab lwm yam. 8. Multi-fluorescence channels: muaj ntau cov fluorescence raws kom paub ntau cov ntawv sau rau tib lub sijhawm.
