Yuav Ua Li Cas Txhim Kho Microscope Resolution
1. Txhim kho daim iav chaw nres nkoj tus nqi;
Dav dav 0.55; N.A1.4, ces R=0.55/2×1.4=0.2 microns
Qhov kev daws teeb meem ntawm lub tshuab microscopes yog 0.2 microns
2. Ua kom luv lub wavelength ntawm ultraviolet lub teeb, ces R=0.27/2 × 1.4=0.1 microns
tuaj yeem muab ob npaug rau qhov kev daws teeb meem (fluorescence microscope)
Siv luv X thiab -rays, lub wavelength ntawm lub electron ntws nyob rau hauv lub electron microscope yog 0.0387A
2. Hloov hom kev txhaj tshuaj ntawm lub tshuab tsom iav, los ntawm ncaj qha mus rau oblique, thiab muaj kev cuam tshuam lub teeb tsom iav hauv qhov tsaus ntuj, muab ob npaug rau kev daws teeb meem, thiab saib lub cev muaj sia nyob.
3. Hloov cov qauv ntawm cov nthwv dej luv luv, nce lub ncov; txo lub trough, siv 1/2 ncov los yog trough, thiaj li los txhim kho qhov kev daws teeb meem, xws li theem contrast microscope. Xyoo 1952, nws yeej Nobel nqi zog. Kev daws teeb meem 0.1 micron (lub cev nyob, qhov zoo thiab qhov tsis zoo sib txawv)
4. Siv 1/4 wavelength los txhim kho kev daws teeb meem, xws li kev cuam tshuam sib txawv ntawm lub tshuab tsom
