Yuav ua li cas xaiv cov fluorescence li cas
Fluorescence tsom kwm yog tus txheem microschopic cov khoom siv hauv chaw soj ntsuam thiab cov chaw pathology, uas siv cov fluorescence flueseCes rau kev soj ntsuam thiab kev tsom. Nws yog dav siv nyob rau hauv ntau daim teb xws li xov tooj ntawm tes, neurobiology, etrobiology, etorication ntawm cov tshuaj lom neeg zoo xws li hloov pauv ntawm ion concentration, thiab li ntawd On.
Xaiv cov fluorescent lub teeb
Cov peev txheej uas nquag siv fluorescent tau tam sim no suav nrog cov teeb pom kev zoo mercurs, hlau halide teeb, thiab kev tsim kho tau sai sai, thiab kev tsim kho tau sai sai LED nyob rau hauv xyoo tas los no. Lub spectrum ntawm fluorescent teeb tau tuaj yeem txuas ntxiv mus tau lossis tsis sib txawv, thiab lub zog sib txawv hauv cov sib txawv. LED teeb tau yog maj los ua lub teeb pom kev zoo vim lawv qhov chaw ruaj khov, thiab kev nyab xeeb ntau dua thiab ib puag ncig kev nyab xeeb.
Kev Nkag Siab
Hauv cov tshuaj fluorescence microscopy kev soj ntsuam, vim muaj kev sib tshooj ntawm cov tshuaj fluorescent, lawv tau khi ua ke. Txawm li cas los xij, cov khoom noj sawv ntxov Epifluorescence tsis tsuas yog sau lub teeb saum toj thiab hauv qab lub dav hlau, ua rau muaj kev daws teeb meem zoo nkauj thiab sib piv.
Kev ua tsis haum tsuas yog kuaj pom cov feem ntawm lub teeb pom los ntawm lub dav hlau focal, yog li daws cov teeb meem saum toj no. Lub hauv paus teeb pom kev ua ib qho me me thiab zoo rau ntawm lub dav hlau hauv lub dav hlau los ntawm cov kab mob poral los ntawm lub dav hlau focal yog sau los ntawm lub hom phiaj lens. Feem ntau ntawm cov fluorescence emitted los ntawm cov ntsiab lus saum toj no lossis qis dua lub dav hlau ntawm lub hom phiaj nkag mus tsis tau tus pinhole. Tsuas yog cov fluorescence nyob ntawm lub dav hlau hauv focal thiab ib feem me me ntawm lub teeb fluorescence tuaj yeem dhau ntawm lub dav hlau sab nraum lub teeb sab hauv ntej lossis sab nraud ntawm nkag mus rau hauv lub ntsuas hluav taws xob los ntawm tus pinhole. Tus kuaj pom cov duab yog daim duab los ntawm lub dav hlau focal, yog li cov duab kawg zoo yog txhim kho.
Vim muaj ntau qhov zoo thiab kev coj ua ntawm laser scanning kev ua kom pom tseeb ntawm kev tshawb fawb ntawm Biology, Botany, thiab Cell kev tshawb fawb, thiab kev tshawb fawb ntawm tes. Nyob rau tib lub sijhawm, nyob rau hauv cov chaw tshawb fawb tshawb fawb yav tom ntej, nws yuav yog qhov cuab yeej tshawb nrhiav thiab tseem ceeb tshaj plaws.
