Yuav ua li cas kho lub laser siv ntawm laser confocal microscope
Muaj ob qhov kev hloov kho ntawm laser siv: lub zog tswj pob qhov rooj thiab Acousto-Optical Tunable Filter (AOTF).
1. Lub zog tswj pob qhov rooj: feem ntau teem rau 30 feem pua ntawm tus nqi loj ntawm * . Nrog rau qhov nce ntawm cov zis, lub neej ntawm laser yuav luv luv, thiab laser suab kuj yuav raug generated. AOTF, PMT, thiab lwm yam yuav tsum tau kho ua ntej kom tau txais kev siv zog zoo.
2. Acousto-optic tunable lim: tswj lub excitation teeb beam thiab siv, thiab tuaj yeem tig los yog tawm lub laser ntawm millisecond ceev los yog kho kom hloov ntawm 0-100 feem pua. Nyob rau hauv txoj kev no, cov kab hluav taws xob sib txawv tuaj yeem siv rau txhua qhov duab taw tes, thiab qhov kev siv yuav tsum tau xaiv.
Lub hauv paus ntsiab lus ua haujlwm: Bragg diffraction nyhuv ntawm lub suab nthwv dej propagating nyob rau hauv ntau yam xov xwm yog siv. Thaum lub teeb liab RF nrog qee zaus yog cov tswv yim, AOTF cuam tshuam qhov teeb meem polychromatic teeb, thiab xaiv lub teeb monochromatic nrog lub wavelength tuaj. Lub wavelength ntawm lub teeb monochromatic muaj ib qho kev sib txuas lus nrog RF zaus f, thiab lub wavelength ntawm cov zis tuaj yeem hloov tau sai thiab randomly los ntawm kev kho cov teeb liab hluav taws xob. AOTF tuaj yeem kho qhov kev siv lub laser tsis tu ncua, thiab muaj kev ua haujlwm ntawm kev hloov kho sai, paub txog kev txhawb nqa hauv zos thiab kev tshuaj ntsuam xyuas, thiab kev hloov pauv ceev wavelength (microsecond qib). Nyob rau hauv laser confocal microscope, photomultiplier tube tsis tuaj yeem ua kom lub laser siv, tab sis tuaj yeem kho qhov photoelectric siv tom qab imaging, yog li qhov ci ntawm cov duab tuaj yeem hloov kho los ntawm kev kho qhov voltage ntawm photomultiplier raj. Tsis tas li ntawd, qhov loj ntawm lub pinhole cuam tshuam rau photon kom muaj nuj nqis thiab tuaj yeem kho qhov ci ntawm daim duab. Lub raj photomultiplier yog lub tshuab nqus tsev tshwj xeeb uas yog qhov tsis tshua muaj siab rau ultraviolet lub teeb, pom lub teeb thiab ze lub teeb infrared. Nws tuaj yeem ua rau cov teeb liab tsis muaj zog kho qhov muag mus rau 108 zaug thiab ua rau qhov ntsuas qhov muag pom.
