Yuav ua li cas kho lub laser siv ntawm lub laser scanning confocal microscope
Muaj ob yam kev hloov kho rau laser siv: lub hwj chim tswj pob qhov rooj thiab acousto -optic tunable lim (AOTF)
1. Lub zog tswj pob qhov rooj: Feem ntau teem rau 30% ntawm tus nqi siab tshaj plaws. Ua kom cov zis ntau ntxiv yuav ua rau lub neej luv luv ntawm lub laser thiab tsim lub suab nrov laser. AOTF, PMT, thiab lwm yam yuav tsum tau kho ua ntej kom tau txais qhov zoo tshaj plaws siv.
2. Acousto optic tunable lim: tswj lub excitation teeb beam thiab siv, thiab tuaj yeem qhib lossis tawm lub laser lossis kho nws ntawm 0-100% hauv milliseconds. Qhov no tso cai rau kev siv cov kab teeb laser sib txawv rau txhua qhov duab taw tes thiab xaiv qhov yuav tsum tau siv zog.
Lub hauv paus ntsiab lus ua haujlwm: Nws siv Bragg diffraction nyhuv ntawm suab nthwv dej propagating nyob rau hauv ntau yam xov xwm ntawm qhov teeb meem Bragg diffraction nyob rau hauv lub propagation nruab nrab. Thaum muaj qee zaus ntawm RF teeb liab tawm tswv yim, AOTF cuam tshuam qhov teeb meem polychromatic teeb thiab xaiv lub teeb monochromatic nrog lub wavelength ntawm cov tswv yim. Lub wavelength ntawm lub teeb monochromatic sib raug rau ib qho - mus rau - ib qho nrog RF zaus f. Ntev npaum li lub teeb liab hluav taws xob tau kho, qhov tso zis wavelength tuaj yeem hloov tau sai thiab hloov pauv. AOTF ua tiav kev hloov kho tas li ntawm laser siv thiab muaj kev hloov kho sai, paub txog kev txhawb nqa hauv zos thiab kev soj ntsuam, kev hloov pauv ceev wavelength (microsecond qib). Nyob rau hauv laser confocal microscopy, cov hlab photomultiplier tsis tuaj yeem ua rau kom muaj zog ntawm laser, tab sis tuaj yeem kho qhov siv ntawm lub teeb pom kev tom qab. Yog li ntawd, los ntawm kev kho qhov voltage ntawm lub photomultiplier raj, lub brightness ntawm daim duab yuav raug kho. Tsis tas li ntawd, qhov loj ntawm focal pinhole cuam tshuam rau photon kom muaj nuj nqis thiab tuaj yeem kho qhov ci ntawm daim duab. Lub raj photomultiplier yog lub tshuab nqus tsev tshwj xeeb uas muaj kev nkag siab zoo rau ultraviolet, pom, thiab ze rau - lub teeb infrared. Nws tuaj yeem txhim kho lub teeb pom kev tsis muaj zog los ntawm 108 zaug, tso cai rau ntsuas lub teeb liab.
