Fluorescence microscopy nrhiav txoj kev
fluorescence microscope
Fluorescence microscopy yog ib qho cuab yeej tseem ceeb rau immunofluorescence cytochemistry. Nws yog tsim los ntawm cov khoom tseem ceeb xws li lub teeb ci, lim phaj system, thiab kho qhov muag. Nws siv qee lub teeb ntawm lub teeb kom zoo siab rau cov qauv thiab emit fluorescence, uas tau nthuav dav los ntawm lub hom phiaj thiab lub qhov muag kom pom cov duab fluorescence ntawm cov qauv (Daim duab 1).
(1) Lub teeb ci
Niaj hnub no, 200W ultra-siab siab mercury teeb feem ntau yog siv los ua lub teeb ci. Nws yog ua los ntawm quartz iav, nrog ib tug kheej kheej zoo nyob rau hauv nruab nrab thiab ib tug npaum li cas ntawm mercury nyob rau hauv. Thaum lub sijhawm ua haujlwm, qhov tso tawm ntawm ob lub electrodes ua rau cov mercury evaporate, thiab lub siab nyob rau hauv lub kheej kheej nce sai. Thaum cov mercury evaporates tag nrho, nws tuaj yeem ncav cuag 50-70 tus qauv atmospheric pressures, uas feem ntau yuav siv li ntawm 5-15 feeb. Lub luminescence ntawm ultra-siab siab mercury teeb yog qhov tshwm sim ntawm kev tawm ntawm electrodes, uas txuas ntxiv dissociates thiab txo cov mercury molecules thiab emits photons. Nws emits muaj zog ultraviolet thiab xiav violet lub teeb, uas yog txaus rau excite ntau yam fluorescent tshuaj, yog li ntawd, nws yog dav siv nyob rau hauv fluorescence microscopes.
Kev ceev faj rau kev siv fluorescence microscope
(1) Ua raws li cov lus qhia ntawm lub Hoobkas fluorescence microscope thiab tsis txhob hloov qhov kev pab cuam arbitrarily.
(2) Kev tshuaj xyuas yuav tsum tau ua hauv chav tsaus. Tom qab nkag mus rau hauv chav tsaus, txuas lub hwj chim thiab teeb lub teeb ci ultra-siab mercury rau 5-15 feeb. Tom qab lub teeb pom kev zoo emits lub teeb muaj zog thiab ruaj khov, lub qhov muag yuav tsum tau hloov mus rau qhov tsaus ntuj ua ntej pib soj ntsuam cov qauv.
(3) Txhawm rau tiv thaiv UV puas rau lub qhov muag, cov iav tiv thaiv yuav tsum tau hnav thaum kho lub teeb ci.
(4) Lub sijhawm tshuaj xyuas yuav tsum yog 1-2 teev txhua lub sijhawm. Yog tias nws tshaj li 90 feeb, qhov luminous siv ntawm ultra-siab siab mercury teeb maj mam txo qis thiab cov fluorescence tsis muaj zog; Cov kab mob raug ultraviolet hluav taws xob rau 3-5 hnub
Tom qab min, fluorescence kuj txo qis; Yog li, lub sijhawm ntev tshaj plaws yuav tsum tsis pub tshaj 2-3 teev.
(5) Lub neej ntev ntawm fluorescent microscope lub teeb pom kev yog txwv, thiab cov qauv yuav tsum tau kuaj xyuas hauv nruab nrab kom txuag tau sijhawm
Txuag sijhawm thiab tiv thaiv lub teeb ci. Thaum huab cua kub, lub kiv cua hluav taws xob yuav tsum tau muab ntxiv kom dissipate cua sov thiab txias. Thaum hloov lub teeb tshiab, lub sijhawm siv yuav tsum tau sau tseg txij thaum pib. Thaum lub teeb raug kaw thiab koj xav siv nws dua, koj yuav tsum tos kom lub teeb kom txias txaus ua ntej igniting nws. Tsis txhob teeb lub teeb ci ntau zaus hauv ib hnub.
(6) Saib xyuas cov qauv tam sim tom qab staining, vim tias cov fluorescence yuav maj mam poob qis thaum lub sijhawm. Yog tias cov qauv khaws cia hauv lub hnab yas polyethylene ntawm 4 degree, nws tuaj yeem ncua lub sijhawm fluorescence attenuation thiab tiv thaiv cov evaporation ntawm tus neeg sawv cev mounting.
(7) Cov txheej txheem txiav txim rau fluorescence brightness feem ntau muab faib ua plaub theem, xws li "ib" - tsis muaj lossis pom tsis muaj zog fluorescence. "+" - Tsuas pom cov fluorescence pom. ++- Pom tau nrog ci fluorescence. "+++" - Pom pom thiab dazzling fluorescence.
