Fluorescence microscope siv txoj hauv kev thiab ceev faj
Txoj kev siv:
(1) Muab cov ntaub ntawv tso rau ntawm tus nqa khoom theem.
(2) Hloov ntawm fluorescence microscope voltage regulator . Tom qab ntawd nias qhov pib pab pawg kom ignite lub UV teeb yuav tsum tsis txhob muab tua rau 15min, thiab yuav tsum tsis txhob rov pib dua rau 3min tom qab nws muab tua.
(3) Tig lub excitation lim rau v, dichroic zaj duab xis rau v, xaiv 495 lossis 475nm thaiv lim.
(4) Xaiv uvFL40, uvFL100 fluorescence txais lub hom phiaj lens microscopy.
(5) Ntxiv cov roj uas tsis muaj fluorescent rau ntawm qhov swb, thawj 40 x, thiab tom qab ntawd 100 x tsom iav tsom iav. Qhia fluorescence.
(7) Vim hais tias cov khoom siv fluorescent los ntawm ultraviolet irradiation nrog kev loj hlob ntawm fluorescence maj mam tsis muaj zog nyob rau lub sij hawm, yog li kev soj ntsuam microscopic yuav tsum nquag hloov qhov kev pom.
(8) kuj tuaj yeem siv uvFL10 lossis uvFL20 cov khoom siv microscopy qis (nrog rau qhov tsis tshua pom zoo yam tsis ntxiv cov roj fluorescent).
Fluorescence duab kaw txoj kev:
Fluorescence duab pom los ntawm fluorescence microscope, ib tug yog nrog morphological yam ntxwv, qhov thib ob yog nrog cov xim thiab brightness ntawm fluorescence, nyob rau hauv judging cov qhabnias, ob yuav tsum tau ua ke nrog ib tug kev txiav txim. Cov txiaj ntsig tau raug kaw raws li cov cim qhia, piv txwv li, los ntawm kev pom kev pom los ntawm tus neeg ua haujlwm. Raws li kev soj ntsuam dav dav, kev ntseeg tau yooj yim. Nrog rau kev txhim kho thev naus laus zis thiab kev tshawb fawb, mus rau qib sib txawv, kev siv lub hom phiaj ntsuas ntsuas los sau cov txiaj ntsig ntawm kev txiav txim, xws li kev siv xov tooj ntawm tes spectrophotometer, duab tsom xam thiab lwm yam cuab yeej. Txawm li cas los xij, cov txiaj ntsig tau sau los ntawm cov cuab yeej no yuav tsum tau ua ke nrog kev txiav txim siab.
Fluorescence microscope yees duab thev naus laus zis yog qhov tsim nyog rau kev sau cov duab fluorescence, vim tias fluorescence yooj yim ploj mus thiab tsis muaj zog, kom khaws cov txiaj ntsig ntawm kev yees duab tam sim. Cov txheej txheem yog qhov pib zoo ib yam li micrography zoo tib yam. Tsuas yog yuav tsum tau siv cov duab thaij duab nrawm xws li ASA200 saum toj no lossis 24. saum toj no. Vim tias lub teeb ci ultraviolet ntawm cov fluorescence tawg, xws li FITC cov cim, nyob rau hauv lub teeb ultraviolet irradiation 30s, fluorescence brightness txo los ntawm 50%. Yog li ntawd, qhov kis ceev yog qeeb heev los ntes cov duab fluorescence. Kev tshawb fawb dav dav fluorescence microscopes muaj semi-automatic lossis tag nrho-automatic microphotography system cov cuab yeej.
Cov kev ceev faj:
(1) Ua haujlwm nruj raws li cov cai ntawm fluorescence microscope Hoobkas phau ntawv, tsis txhob hloov cov txheej txheem arbitrarily.
(2) Kev tshuaj xyuas yuav tsum tau ua hauv chav tsaus. Tom qab nkag mus rau hauv chav tsaus, txuas cov khoom siv hluav taws xob, teeb lub teeb hluav taws xob ultra-siab mercury rau 5-15min, tos kom txog thaum lub teeb pom kev zoo emits lub teeb muaj zog kom ruaj khov, thiab lub qhov muag tau hloov mus rau qhov tsaus ntuj, thiab ces pib soj ntsuam tus qauv.
(3) Txhawm rau tiv thaiv kev puas tsuaj rau lub qhov muag los ntawm lub teeb ultraviolet, cov iav tiv thaiv yuav tsum tau hnav thaum kho lub teeb ci.
(4) xyuas lub sij hawm txhua lub sij hawm mus rau 1 ~ 2h yog qhov tsim nyog, ntau tshaj 90min, ultra-high-pressure mercury teeb luminous siv maj mam txo, fluorescence tsis muaj zog; cov qauv irradiated los ntawm ultraviolet lub teeb 3 ~ 5min, fluorescence kuj tseem tsis muaj zog; yog li ntawd, * yuav tsum tsis txhob ntau tshaj 2 ~ 3h.
(5) fluorescence microscope lub teeb lub neej yog txwv, cov qauv yuav tsum tau soj ntsuam hauv nruab nrab, txhawm rau txuag lub sijhawm thiab tiv thaiv lub teeb ci. Thaum nws kub, yuav tsum tau ntxiv cov kiv cua txias, lub teeb tshiab yuav tsum tau sau tseg txij thaum pib siv lub sijhawm. Thaum lub teeb raug tua thiab xav siv dua, lub teeb yuav tsum tau txias txaus ua ntej teeb pom kev zoo. Lub teeb ci yuav tsum zam ntau zaus.
(6) Saib xyuas cov qauv tam sim tom qab staining, vim tias cov fluorescence yuav maj mam ploj zuj zus mus. Yog tias cov qauv muab tso rau hauv ib lub hnab yas polyethylene khaws cia ntawm 4 degree, nws tuaj yeem ncua lub sijhawm ntawm fluorescence tsis muaj zog thiab tiv thaiv cov evaporation ntawm tus neeg sawv cev sealing.
(7) Cov txheej txheem txiav txim rau fluorescence ci ntsa iab: feem ntau muab faib ua plaub theem, uas yog, 'ib' - tsis muaj lossis pom tsis muaj zog fluorescence. '+' - tsuas yog pom tseeb fluorescence. '+ +' - pom nrog lub teeb ci
