Fluorescence microscope pib ua haujlwm
ib. Tua lub teeb nyob rau hauv chav tsev thiab tig rau lub tshuab hluav taws xob mercury teeb;
b. Xaiv cov lim zoo raws li cov fluorescein sau los ntawm cov qauv;
c. Tso tus qauv thiab nrhiav qhov chaw tsim nyog saib;
d. Yog tias koj xav tau cov duab, thov paub meej tias cov xim zaj duab xis tau teeb tsa hauv lub koob yees duab;
e. Tig rau lub tshuab Selfie, xaiv lub iav phau ntawv, feem ntau qhov kev tua ceev yog nyob rau hauv 0.5-10 vib nas this;
f. Tom qab siv, tua tag nrho cov hwj huam thiab ua cov ntaub ntawv siv. tus
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3. Cov txheej txheem kaw cov duab fluorescence
Cov duab fluorescence pom los ntawm fluorescence microscope muaj ob qho tib si morphological yam ntxwv thiab fluorescence xim thiab ci. Thaum txiav txim qhov tshwm sim, ob qho tib si yuav tsum tau ua ke rau kev txiav txim siab. Cov txiaj ntsig tau raug kaw raws li cov cim qhia, uas yog, kev pom kev pom los ntawm cov neeg ua haujlwm. Raws li kev soj ntsuam dav dav, kev ntseeg tau yooj yim. Nrog rau kev txhim kho thev naus laus zis thiab kev tshawb fawb, cov ntsuas lub hom phiaj yog siv los sau cov txiaj ntsig kev txiav txim siab mus rau qib sib txawv, xws li xov tooj ntawm tes spectrophotometers, duab tsom xam thiab lwm yam cuab yeej. Txawm li cas los xij, cov txiaj ntsig tau sau los ntawm cov cuab yeej no yuav tsum tau ua ke nrog cov kev txiav txim siab. tus
Fluorescence microscope photography yog qhov tsim nyog rau kev sau cov duab fluorescence. Txij li cov fluorescence yooj yim ploj mus thiab tsis muaj zog, nws yog ib qho tsim nyog los sau cov txiaj ntsig ntawm lub sijhawm. Cov txheej txheem yog qhov pib zoo ib yam li cov duab photomicrography. Tsuas yog yuav tsum tau siv high-speed photosensitive zaj duab xis. Vim tias lub teeb ci ultraviolet muaj qhov cuam tshuam zoo rau fluorescence quenching, xws li FITC cov cim, cov fluorescence brightness yuav txo los ntawm 50 feem pua thaum irradiated nrog ultraviolet lub teeb rau 30s. Yog li ntawd, yog tias qhov kis tau nrawm dhau lawm, cov duab fluorescent tsis tuaj yeem nqa. Kev tshawb fawb dav dav fluorescence microscopes muaj semi-automatic lossis tag nrho tsis siv neeg photomicrography system li. tus
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4. Kev ceev faj rau kev siv Fluorescence Microscopy
ib. Ua haujlwm nruj me ntsis raws li qhov yuav tsum tau ua ntawm fluorescence microscope Hoobkas cov lus qhia, thiab tsis txhob hloov qhov kev pab cuam ntawm lub siab nyiam. tus
b. Kev tshuaj xyuas yuav tsum tau nqa tawm hauv chav tsaus. Tom qab nkag mus rau hauv chav tsaus, txuas cov khoom siv hluav taws xob thiab teeb lub teeb ci ultra-siab mercury rau 5-15 feeb. Tom qab lub teeb muaj zog los ntawm lub teeb pom kev ruaj khov, lub qhov muag tau hloov mus rau qhov tsaus ntuj, thiab tom qab ntawd pib soj ntsuam cov qauv. tus
c. Txhawm rau tiv thaiv kev puas tsuaj ntawm ultraviolet rays rau lub qhov muag, hnav tsom iav tiv thaiv thaum kho lub teeb ci. tus
d. Lub sijhawm tshuaj xyuas zoo dua yog 1-2 teev txhua lub sijhawm. Yog tias nws tshaj li 90 feeb, qhov luminous siv ntawm ultra-siab siab mercury teeb yuav maj mam txo thiab cov fluorescence yuav tsis muaj zog; tom qab cov qauv raug irradiated nrog ultraviolet lub teeb rau 3-5 feeb, cov fluorescence kuj yuav tsis muaj zog heev; yog li ntawd, nws yuav tsum tsis txhob ntau tshaj 2-3 teev. . tus
e. Lub neej ntawm fluorescent microscope lub teeb qhov chaw yog txwv, thiab cov qauv yuav tsum tau kuaj xyuas hauv nruab nrab kom txuag tau sijhawm thiab tiv thaiv lub teeb ci. Thaum huab cua kub, cov kiv cua yuav tsum tau ntxiv kom dissipate tshav kub, thiab cov qij tshiab yuav tsum tau sau tseg txij thaum pib ntawm lawv lub sijhawm siv. Thaum koj xav siv nws dua tom qab lub teeb raug tua, koj yuav tsum tau tos kom txog thaum lub teeb txias txaus ua ntej teeb pom kev zoo. Tsis txhob teeb lub teeb ci ntau zaus hauv ib hnub. tus
f. Saib xyuas cov qauv tam sim tom qab staining, vim tias cov fluorescence yuav maj mam tsis muaj zog thaum lub sijhawm. Yog tias cov qauv khaws cia rau hauv lub hnab yas polyethylene ntawm 4 degree, lub sijhawm fluorescence tsis muaj zog tuaj yeem ncua sijhawm thiab tus neeg sawv cev tuaj yeem tiv thaiv kom tsis txhob ya mus. tus
g. Kev txiav txim tus qauv ntawm fluorescence brightness: nws yog feem ntau muab faib ua plaub theem, uas yog, "-" txhais tau tias tsis muaj los yog tsis muaj zog fluorescence yuav pom. "ntxiv" txhais tau hais tias tsuas yog pom meej fluorescence pom. " plus plus " txhais tau hais tias ci fluorescence pom. "plus plus plus" txhais tau hais tias pom qhov kaj fluorescence.
