(Qhov txawv ntawm (ob-photon, confocal) fluorescence microscopes thiab cov microscopes zoo tib yam
Fluorescence microscope yog kev siv lub teeb ci ultraviolet los ua lub teeb ci, siv los irradiate cov khoom los kuaj xyuas, kom cov khoom emits ib lub teeb ci, thiab tom qab ntawd kev soj ntsuam ntawm cov khoom yog nqa tawm nyob rau hauv lub microscope. Nws yog tsuas yog siv rau immunofluorescence hlwb, uas yog tsuas yog tsim los ntawm lub teeb qhov chaw, lim phaj system thiab kho qhov muag system los soj ntsuam cov fluorescence duab ntawm cov qauv los ntawm lub magnification ntawm eyepiece thiab lub hom phiaj lens. Cia peb saib ntawm lub fluorescence microscope thiab qhov zoo tib yam optical microscope muaj qhov txawv li cas.
1, nyob rau hauv illumination hom
Lub illumination ntawm fluorescence microscope feem ntau yog nrog cov ntog hom, uas yog hais tias, lub teeb qhov chaw yog los ntawm lub hom phiaj lens rau muab tso rau hauv lub xeem qauv.
2, hauv kev daws teeb meem
Fluorescence microscope siv lub teeb ultraviolet ua lub teeb ci, lub wavelength luv luv, tab sis qhov kev daws teeb meem yog siab dua li qub optical microscope.
3, qhov sib txawv ntawm cov lim
Fluorescence microscope yog siv ob lub lim dej tshwj xeeb, nyob rau hauv pem hauv ntej ntawm lub teeb ci yog siv los lim tawm lub teeb pom kev, nruab nrab ntawm lub hom phiaj lens thiab lub qhov muag kom lim tawm lub teeb ultraviolet, uas tuaj yeem tiv thaiv tib neeg lub qhov muag.
Fluorescence microscope kuj belongs rau ib hom ntawm optical microscope, feem ntau fluorescence microscope excitation wavelength yog luv, yog li qhov no coj mus rau lub fluorescence microscope thiab zoo tib yam microscope lwm yam qauv tsim thiab siv sib txawv, fluorescence microscope feem ntau muaj zoo capture ntawm lub teeb tsis muaj zog, yog li nyob rau hauv qhov tsis muaj zog fluorescence, nws muaj peev xwm imaging thiab zoo. Ua ke nrog kev txhim kho txuas ntxiv ntawm fluorescence microscope nyob rau xyoo tas los no, lub suab nrov kuj tseem txo qis. Yog li ntawd, ntau thiab ntau fluorescence microscopes tau siv.
Ob-photon fluorescence microscope muaj ntau yam zoo:
(1) Ntev wavelengths ntawm lub teeb tsis tshua muaj kev cuam tshuam los ntawm kev tawg dua li luv wavelengths ntawm lub teeb yooj yim nkag mus rau hauv cov qauv;
(2) Cov fluorescent molecules sab nraum lub dav hlau focal tsis zoo siab kom lub teeb excitation ntau tuaj yeem ncav cuag lub dav hlau focal, kom lub teeb pom kev tuaj yeem nkag mus tob rau hauv cov qauv;
3) Ntev wavelengths ntawm ze-infrared teeb tsis muaj tshuaj lom rau cov hlwb dua li luv wavelengths;
4) Thaum siv ob-photon microscope los soj ntsuam tus qauv, photobleaching thiab phototoxicity tsuas yog nyob ntawm lub dav hlau focal. Yog li ntawd, ob-photon microscopes yog qhov tsim nyog tshaj li ib-photon microscopes rau kev soj ntsuam cov qauv tuab, rau kev soj ntsuam cov cell nyob, los yog ua qhov kev sim photobleaching.
