Qhov sib txawv ntawm fluorescence microscopy
Cov tsom iav fluorescence
1. Fluorescence microscope uses ultraviolet light as a light source to irradiate the object being inspected, causing it to emit fluorescence, and then observe the shape and position of the object under the microscope. Fluorescence microscopy yog siv los kawm qhov nqus, thauj, faib khoom, thiab cov tshuaj hauv zos hauv cov tshuaj. Qee cov tshuaj hauv cov qog, xws li chlorophyll, tuaj yeem fluoresce thaum raug rau ultraviolet hluav taws xob; Tseem muaj qee yam tshuaj uas tsis tuaj yeem tawm fluorescence lawv tus kheej, tab sis kuj tseem tuaj yeem tso cov tshuaj tsw qab lossis ua kom muaj cov tshuaj lom fluorescodies thiab irradiated nrog ultraviolet teeb. Fluorescence microscopy yog ib qho ntawm cov cuab yeej rau cov kev tshawb fawb thiab kev tshawb fawb kom pom tseeb ntawm cov tshuaj no.
2. Lub ntsiab cai ntawm fluorescence tsom iav:
(A) Qhov Chaw Ntsuas Lub Teeb: Lub hauv paus teeb pom kev ntawm ntau yoj yoj (los ntawm ultraviolet mus rau infrared).
(B) Kev zam lub teeb ci lim: Xa cov nthwv dej tshwj xeeb uas tuaj yeem ua rau fluorength ci, thaum thaiv lub teeb uas tsis muaj txiaj ntsig zoo rau cov fluorescence.
(C) Cov yam ntxwv fluorescent: feem ntau tsuas nrog cov xim fluorescent.
(D) Kev thaiv cov lim: nws xaiv tau transmits fluorescence los ntawm cov quav dev, thiab ib co wavelengths hauv fluorescence kuj tseem xaiv tau kis. Lub tsom iav tsom uas siv cov teeb ci ultraviolet ua lub teeb ci kom ua rau qhov chaw ua kom zoo nkauj tshwm sim fluorescence. Lub tshuab hluav taws xob hauv hluav taws xob tau sib sau ua ntej los ntawm Knorr thiab Haruster siv hluav taws xob hluav taws xob ceev dua tshiab hloov chaw teeb ci. Vim tias cov wavelength ntau ntawm cov hluav taws xob ntws piv rau lub teeb hluav taws xob tau mus txog 8}}}}}}}}}}}}}}}}}}}}}}}}}}}}}} Lub scanning Electron tsom iav, uas tau siv thawj zaug hauv xyoo 1963, tso cai rau tib neeg pom cov qauv me me ntawm cov khoom ntawm cov khoom.
3. Daim ntawv thov tau txais: Siv cov duab loj ntawm cov khoom me me. Feem ntau siv rau kev soj ntsuam ntawm biology, tshuaj, microscopic hais, thiab lwm yam.
Kev Nkag Siab
1. Kev Nkag Siab rau lub tsom iav ntxiv cov lus qhia txog ib nrab lens txog lub teeb ci ntsa iab uas tau dhau los ntawm lub lens hauv lwm cov lus qhia. Ntawm nws lub focal point, muaj ib qho baffle nrog tus porthole nyob rau ntawm lub focal point, thiab tom qab lub baffle yog tus ntaus phev yog tus phofficultiplitter raj. Nws tuaj yeem xav tias lub teeb pom kev zoo ua ntej thiab tom qab kev tshawb pom lub teeb tsis tuaj yeem tsom mus rau lub qhov system me me thiab yuav raug thaiv los ntawm baffle. Yog li lub photometer ntsuas qhov kev cuam tshuam lub teeb ntawm lub focal point.
2. Lub hauv paus ntsiab lus: Mas taws tsom kwm siv cov teb teeb tsa, thiab cov duab ntawm txhua kis ntawm cov quav dev yog cuam tshuam los ntawm cov ntsiab lus sib txawv; Laser Scanne tsom iav tsom iav rau lub teeb laser kom pom lub teeb ci ntsa iab rau kev tshawb xyuas txhua lub sijhawm ntawm lub dav hlau ntawm lub dav hlau. Qhov chaw nres pom ntawm cov hnoos qeev yog cov kev kuaj pom ntawm qhov pinhole, thiab tau txais kev kuaj pom tus kheej (CCCD) Lub Illumination pinhoination pinhole thiab kev nrhiav kom tau pinhole yog conjugate tus txheeb ze rau lub dav hlau focal ntawm lub hom phiaj lens. Cov ntsiab lus ntawm lub dav hlau hauv qab yog ib txhij tsom rau lub istination pinhole thiab cov ntsiab lus pephanole, thiab cov ntsiab lus sab nraum lub tsom iav sab nraud yuav tsis pom ntawm kev kuaj pom kev pom. Lub txiaj ntsig tawm tswv yim yog qhov kho qhov muag hla ntawm cov hnoos qeev, kov yeej qhov tsis zoo ntawm cov duab dav dav dav hlau.
