Qhov sib txawv ntawm ob-photon microscopy thiab laser confocal microscopy
Laser confocal microscope yog ib txheej ntawm kev soj ntsuam, tsom xam thiab tso zis system siv laser raws li lub teeb qhov chaw, conjugate tsom lub hauv paus ntsiab lus thiab ntaus ntawv nyob rau hauv lub hauv paus ntawm ib txwm optical microscope, thiab cov duab ua ntawm cov khoom pom siv computer. Lub kaw lus tseem ceeb suav nrog lub teeb pom kev zoo laser, lub tshuab tsom iav tsis siv neeg, scanning module (xws li confocal optical channel thiab pinhole, scanning daim iav, detector), digital signal processor, computer thiab duab tso zis khoom (saib, xim tshuab luam ntawv) thiab hais txog. Nrog lub laser scanning confocal microscope, cov qauv kev soj ntsuam tuaj yeem ua kom pom thiab duab. Yog li ntawd, peb-dimensional spatial qauv ntawm cov hlwb tuaj yeem soj ntsuam thiab txheeb xyuas yam tsis muaj kev puas tsuaj.
Nyob rau tib lub sijhawm, laser scanning confocal microscopy yog lub cuab yeej muaj zog rau kev soj ntsuam ntawm cov hlwb nyob, ntau lub cim immunofluorescence thiab ion fluorescence labelling. Qhov xwm txheej ntawm cov spectrum raug tshuaj xyuas meej, thiab cov cim qhia ntawm cov cim sib txawv nrog kev sib tshooj emission spectrum yog qhov txawv.
Qhov tseem ceeb tshaj plaws, rau ntau xim fluorescence staining, nws tshem tawm tag nrho cov nyhuv ntawm fluorescence crosstalk thaum txo qhov poob ntawm fluorescence teeb liab los ntawm cov qauv. Tag nrho cov no yog dhau qhov ncav cuag ntawm lub teeb pom kev dav dav.
