Qhov sib txawv ntawm fluorescence thiab laser confocal microscopy
Cov ntsiab cai txawv
1, fluorescence microscope: ultraviolet lub teeb ua lub teeb ci, siv los irradiate cov khoom los kuaj xyuas, kom nws tawm fluorescence, thiab tom qab ntawd saib cov duab ntawm cov khoom thiab nws qhov chaw nyob hauv lub tshuab tsom.
2, laser confocal microscope: nyob rau hauv lub fluorescence microscope duab nyob rau hauv lub hauv paus ntawm kev sib ntxiv ntawm laser scanning ntaus ntawv, siv lub teeb ultraviolet los yog pom lub teeb los txhawb lub fluorescent sojntsuam.
Cov yam ntxwv sib txawv
1, fluorescence microscope: siv los kawm txog kev nqus ntawm cov khoom hauv cell, thauj, faib thiab thaj chaw ntawm cov tshuaj lom neeg. Qee yam khoom hauv lub cell, xws li chlorophyll, tuaj yeem fluoresce tom qab irradiation los ntawm lub teeb ultraviolet; Qee lwm yam khoom nws tus kheej tsis tuaj yeem fluoresce, tab sis yog stained nrog fluorescent dyes lossis fluorescent antibodies, fluorescence kuj tuaj yeem fluoresce los ntawm ultraviolet irradiation.
2, Laser confocal microscope: Siv lub khoos phis tawj rau kev ua duab, thiaj li tau txais cov duab fluorescence ntawm sab hauv microstructure ntawm cov hlwb lossis cov ntaub so ntswg, nrog rau cov qib subcellular los soj ntsuam xws li Ca2+, pH, membrane muaj peev xwm thiab lwm yam physiological signals thiab cellular morphology hloov.
