Piav qhia meej txog kev xaiv cov kauj ruam thiab cov txheej txheem ntawm fluorescent probes rau confocal microscopy
Hauv kev sim nrog confocal microscopes, qee qhov kev sim ntsuas tuaj yeem kuaj pom siv autofluorescence ntawm tus qauv nws tus kheej lossis cov tshuaj exogenous, tab sis feem ntau ntawm cov tshuaj uas yuav tsum tau kuaj tsis muaj fluorescence ntsuas thiab yuav tsum tau sau nrog fluorescent probes. Nws muaj qhov ntsuas fluorescent teeb liab. Yog li, yuav ua li cas xaiv fluorescent sojntsuam rau confocal microscopy?
1. Txiav txim siab cov ntsuas ntsuas raws li lub hom phiaj ntawm kev sim
Thaum qhov ntsuas qhov ntsuas tau txiav txim siab, qhov kev xaiv ntau yam ntawm fluorescent probes kuj tau txiav txim siab. Nws tuaj yeem pom tau tias qhov kev xaiv ntawm qhov kev sojntsuam fluorescent siv rau hauv qhov kev sim yog txiav txim siab los ntawm lub hom phiaj ntawm qhov kev sim. Piv txwv li, yog tias lub hom phiaj ntawm qhov kev sim yog los kawm txog kev hloov pauv ntawm calcium ions hauv cardiomyocytes thaum atrial fibrillation tshwm sim, nws tuaj yeem txiav txim siab tias cov tshuaj uas yuav tsum tau kuaj yog calcium ions hauv cardiomyocytes, thiab kev xaiv ntau yam ntawm fluorescent probes yog txwv rau. calcium ion probes uas tuaj yeem sau cov hlwb muaj sia. Koob.
2. Txheeb xyuas qhov ntau ntawm cov khoom siv fluorescent
Rau kev sim tshuaj tshiab lossis cov ntsuas ntsuas, yog tias lub hom phiaj ntawm qhov kev sim tsis yog los tshawb nrhiav cov tshuaj fluorescent tshiab, koj tuaj yeem xaiv siv cov kev tshawb fawb paub tab thiab cov ntawv sau npe. Hom lossis ntau yam ntawm fluorescent sojntsuam uas tuaj yeem sau cov tshuaj uas yuav tsum tau kuaj tuaj yeem txiav txim siab los ntawm kev tshawb nrhiav cov ntaub ntawv pov thawj lossis cov khoom lag luam ntawm cov tuam txhab reagent paub zoo hauv tsev thiab txawv teb chaws.
3. Tshawb xyuas seb cov yam ntxwv ntawm cov tshuaj fluorescent puas tau raws li qhov yuav tsum tau ua rau kev npaj cov qauv fluorescent
Cov yam ntxwv ntawm fluorescent probes ncaj qha cuam tshuam rau daim ntawv lo ntawm cov qauv thiab cov txiaj ntsig kev sim. Yog li ntawd, ua ntej xaiv fluorescent probes thiab sau cov qauv hauv qhov kev sim, nws yog ib qho tsim nyog yuav tsum nkag siab tag nrho lawv cov yam ntxwv fluorescence. Cov yam ntxwv ntawm fluorescent probes yuav tsum tau soj ntsuam muaj xws li:
1. Cov yam ntxwv ntawm kev sojntsuam lub teeb thiab cov qauv
2. rhiab heev thiab fluorescence siv fluorescent probes
3. Spectral zog ntawm cov qauv sau nrog fluorescent probes
4. Kev ruaj ntseg ntawm fluorescence
5. Kev sib cuam tshuam ntawm ntau yam fluorescence hauv cov qauv
