Confocal Microscope Kev Ceeb Toom thiab Kev Saib Xyuas
Confocal microscopy tuaj yeem ntsuas ntau yam qauv. Cov no suav nrog cov khoom siv roj ntsha, cov ntaub so ntswg (seem), cellular (subcellular) cov qauv thiab lwm yam. Qhov chaw ntawm fluorescence nyob rau hauv cov qauv yog raws li nram no: autofluorescence, fluorescence staining, fluorescence, fluorescent protein, induced fluorescence (induced fluorescence) thiab enzyme fluorescence (enzymatically ua fluorescence) thiab hais txog. Qhov excitation thiab emission wavelengths ntawm feem ntau ntawm cov fluoresceins tuaj yeem pom nyob rau hauv cov ntaub ntawv dyeing cov tsev qiv ntawv ntawm cov software uas tuaj nrog lub ntsuas.
Confocal microscope ntsuas ntsuas
Laser:
UV laser: 405 nm, lub zog> 30 mW;
Ar-ion laser: 458/476/488/496/514 nm, hwj chim> 50 mW;
Dawb laser: pulsed laser, 470-670 nm, hwj chim Ntau tshaj los yog sib npaug rau 1 mW, kauj ruam 1 nm, 8 excitation spectral kab yuav siv tau tib lub sij hawm, mem tes zaus 80 MHz -10 MHz adjustable.
Lub Hom Phiaj Lens:
Flat field complex achromatic confocal tshwj xeeb fluorescence lub hom phiaj lens.
Ntes:
Ob lub PMT thiab HyD ntes txhua, ib qho yog HyD SMD ntes nrog ib qho kev tshawb nrhiav molecule.
Image Acquisition Processing:
LAS X software (saib ua ntej, sib cais sib cais, jigsaw, multipoint tau txais, kev txheeb xyuas cov ntaub ntawv), SymPho Lub Sijhawm 64 (lub neej ua duab, kev tsom xam cov ntaub ntawv), Huygens Professional (duab deconvolution processing, visualization thiab tsom xam, thiab lwm yam).
Live cell workstation:
Kub tswj + CO2 premixing.
Confocal microscope ceev faj thiab tu
Khaws kom deb ntawm qhov chaw ntawm hluav taws xob hluav taws xob nyob ib ncig ntawm lub ntsuas;
Ib puag ncig tsis muaj kev vibration thiab muaj zog huab cua cuam tshuam;
Lub chamber muaj cov txheej txheem shading los xyuas kom meej tias cov qauv fluorescent yuav tsis bleached los ntawm exogenous lub teeb;
Ib puag ncig huv;
Tswj kev ua haujlwm kub ntawm 5-25 degree .
