Txoj kev ntxuav tom qab ntsuas cov khoom noj siv mis nrog pH meter
Muaj ntau ntau hom kev tu rau acidity / pH meters. Thaum ntsuas cov xov xwm sib txawv lossis ua haujlwm nyob rau hauv ib puag ncig sib txawv, kev sib kis ntawm pH meter / pH meter electrode sib txawv, yog li txoj kev ntxuav yuav raug tshuaj xyuas los ntawm rooj plaub. Kab lus no piav qhia yuav ua li cas ntxuav cov khoom siv mis nyuj tom qab ntsuas lawv cov acidity siv pH meter, thiab yuav ua li cas ntxuav lawv rau cov protein ntau thiab cov protein ntau. Nco ntsoov them sai sai rau lub sijhawm ua haujlwm, raws li iav pH / pH meter electrodes feem ntau yog siv los ntsuas cov tshuaj no. Ntxuav cov sediment sab laug ntawm lub electrode:
1) Thaum kuaj cov mis nyuj muaj protein ntau:
So hauv hydrochloric acid tov ntawm gastric protease (HCl concentration ntawm 0.1mol/L) rau 15 feeb, los yog tsau nyob rau hauv 10% sodium hypochlorite tov rau 5 feeb. Tom qab ntawd, ntxuav nrog dej ntshiab thiab tsau rau hauv 3.3mol / L KCL electrode soaking tov rau 12 teev kom muaj peev xwm sib npaug ntawm electrode. Qhov no tuaj yeem rov qab ua nws cov haujlwm qub. Yog tsis yog, nws nyob ntawm seb puas yuav hloov cov electrode;
2) Thaum kuaj cov protein ntau thiab cov khoom noj muaj roj ntau:
Soak lub acidity / pH meter electrodes hauv cov tshuaj ntxuav tais diav rau 1 teev. Tom qab tshem tawm cov electrode, yaug nws kom huv si thiab muab tso rau hauv lub plab protease tov rau 4 teev. Txoj kev npaj rau gastric protease tov: Sib tov 5% gastric protease 0.5MOL / L hydrochloric acid tov nyob rau hauv ib tug ratio ntawm 1: 1 * Tom qab ntawd, tshem tawm cov electrode, ntxuav nws nrog distilled dej, thiab tsau nyob rau hauv ib tug 3mol. /L (yog 3.3mol) KCL tov rau tom qab siv. Cov electrode yuav tsum tau ntim nrog 3MOL / L KCL tov kom tsis txhob qhuav
Vim tias acidity / pH ntsuas tau siv dav hauv kev lag luam mis nyuj. Tshwj xeeb tshaj yog nyob rau hauv kev ntsuam xyuas ntawm ib nrab thiab tiav cov khoom, nws plays lub luag hauj lwm tseem ceeb, thiab xws li pH qhov tseem ceeb qhia txog qhov zoo ntawm cov tshuaj ntsuam xyuas. Yog tias tus nqi pH tsis tuaj yeem ntsuas tau raws sijhawm rau kev siv khoom siv, nws yuav muaj kev cuam tshuam tsis zoo rau kev tsim khoom.
Cov khoom noj mis nyuj muaj cov yam ntxwv ntawm cov protein ntau thiab cov rog, uas tuaj yeem yooj yim ua rau muaj kev cuam tshuam ntawm electrode iav pob capillary. Ib qho electrode nyob rau hauv ib txwm siv yuav muaj kev teb qeeb thiab kev nyeem tsis ruaj khov tom qab peb lub hlis; Txhawm rau txuag cov nqi siv thiab faib cov peev txheej tsim nyog.
