Calibration ntawm PH ' meter ' thiab configuration ntawm tus txheej txheem buffer tov
1. Dab tsi yog tus qauv buffer tov ntawm pH meter?
Cov tshuaj pH tsis yog cov tshuaj uas tuaj yeem ua kom tus nqi pH ruaj khov. Yog hais tias ib qho me me ntawm cov kua qaub los yog alkali ntxiv rau cov tshuaj no, los yog ib qho me me ntawm cov kua qaub los yog alkali yog tsim los ntawm cov tshuaj tiv thaiv hauv cov tshuaj, thiab cov tshuaj yog diluted kom zoo, tus nqi pH ntawm cov tshuaj no yog qhov ruaj khov. Cov tshuaj no tuaj yeem tiv taus ib qho me me ntawm cov kua qaub los yog alkali lossis dilution, yog li ntawd tus nqi pH tsis yooj yim hloov yog hu ua pH tsis haum.
Cov txheej txheem tsis haum ntawm pH meter muaj cov yam ntxwv hauv qab no:
(1) Tus nqi pH ntawm cov qauv kev daws teeb meem yog paub thiab ncav cuag qhov tseeb;
(2) Tus nqi pH ntawm cov txheej txheem kev daws teeb meem muaj qhov zoo rov ua dua thiab ruaj khov, thiab muaj peev xwm tsis muaj peev xwm loj, tus nqi me me dilution thiab qhov kub me me coefficient;
(3) txoj kev npaj ntawm kev daws yog yooj yim;
2. Rau PH meter, lub luag haujlwm tseem ceeb ntawm pH tsis yog dab tsi?
(1) Calibrate pH meter ua ntej ntsuas pH.
⑵ Siv los txheeb xyuas qhov tseeb ntawm pH meter. Piv txwv li, tom qab ntsuas pH ntsuas pH6 nrog pH6.86 thiab pH4.00, ntxig pH electrode rau hauv pH9.18 tshuaj xyuas seb tus nqi tso tawm ntawm qhov ntsuas puas zoo ib yam nrog tus nqi pHs. ntawm cov qauv kev daws teeb meem;
⑶ Tshawb xyuas seb lub ntsuas pH puas yuav tsum tau rov ntsuas dua thaum ntsuas qhov tseeb. Tom qab pH ntsuas ntsuas tau ntsuas thiab siv, nws yuav ntws los yog hloov pauv. Yog li ntawd, ua ntej kev sim, ntxig cov electrode rau hauv tus qauv tsis nyob ze rau qhov kev daws teeb meem yuav tsum tau sim, thiab txiav txim siab seb nws puas tsim nyog los calibrate dua raws li qhov yuam kev.
⑷ Kuaj qhov ua tau zoo ntawm pH electrode.
3. Yuav ua li cas npaj pH txheem tsis tov?
Rau kev ntsuas pH dav dav, ua tiav cov pH tsis zoo reagents (250ml tuaj yeem npaj tau) tuaj yeem siv. Thaum npaj cov tshuaj, dej deionized yuav tsum tau siv thiab boil rau 15 ~ 30 feeb ua ntej kom tshem tawm cov pa roj carbon dioxide yaj. Txiav qhib lub hnab yas, ncuav lub reagent rau hauv lub beaker, yaj nws nrog tus nqi ntawm cov dej deionized, yaug lub hnab ntim, ncuav rau hauv 250ml volumetric hwj, dilute nws mus rau scale, thiab co nws zoo.
4. Yuav ua li cas khaws cia thiab siv cov tshuaj pH tsis zoo;
Tom qab npaj cov tshuaj tsis haum, nws yuav tsum tau ntim rau hauv lub khob iav los yog lub raj mis polyethylene (alkaline pH buffers, xws li pH9.18, pH10.01 thiab pH12.46, yuav tsum tau ntim rau hauv polyethylene fwj). Lub hau lub raj mis yuav tsum tau kaw nruj nreem thiab khaws cia hauv lub tub yees ntawm qhov kub qis (5 ~ 10 degree). Feem ntau, nws tuaj yeem siv tau li ob lub hlis. Yog tias pom muaj turbidity, mildew lossis nag lossis daus, nws siv tsis tau ntxiv lawm. Thaum siv, ob peb 50ml polyethylene vials yuav tsum tau npaj, thiab cov tshuaj tsis nyob rau hauv lub vial loj yuav tsum tau nchuav rau hauv lub vial, thiab sab laug ntawm qhov kub thiab txias rau 1-2 teev ua ntej siv. Tom qab siv, cov tshuaj tsis nyob hauv lub vial tuaj yeem siv rau 2 ~ 3 hnub nyob rau hauv ib puag ncig ntawm> 10 degree. Feem ntau, lub sijhawm siv ntawm peb qhov kev daws teeb meem ntawm pH7.00, pH6.86 thiab pH4.00 tuaj yeem ntev dua, thiab pH tus nqi ntawm cov tshuaj pH9.18 thiab pH10. .01 kuj yooj yim hloov vim absorbing CO2 hauv huab cua.
