Biological microscopy ntawm lub ntim ntawm cov ntaub so ntswg blocks
Biomicroscopy Txog niaj hnub no, cov dej khov-txias fixation, khov ultra-thin seem thiab khov qhuav manipulation yog niaj hnub txoj kev rau x-ray microfields ntawm cov ntaub so ntswg thiab hlwb. Cov ntsiab lus ntawm txoj kev tau piav qhia hauv cov ntsiab lus hauv qab no:
Biological microscopes Microscopes nrog lub condenser lens tuaj yeem txav mus los kom ua tiav qhov ci nruab nrab, lossis qhov aperture ntawm qhov sib txawv ntawm lub teeb nthuav dav tuaj yeem hloov pauv kom ua tiav qhov nruab nrab 9b ci ntsa iab. Yog tias lub teeb ci hnub ci, qhov pom qhov pom tau tuaj yeem tsa tau tsim nyog, thiab qhov aperture ntawm lub teeb hloov pauv tuaj yeem nthuav dav kom tsim nyog. Yog hais tias lub teeb muaj zog heev, ces lub tsom teeb tsom iav tuaj yeem tsim nyog, qhov aperture ntawm lub teeb hloov pauv hloov pauv kom tsim nyog. Yog hais tias nyob rau hauv cov ntaub ntawv no tseem xav tias dig muag, ces koj tuaj yeem xaiv cov lim uas tsim nyog tso rau hauv lub bracket hauv qab qhov tsom teeb. Qhov no tuaj yeem tau los ua kom koj txaus siab rau qhov ci ntsa iab. Ntawm chav kawm, nyob rau hauv kev hloov kho ntawm lub Upper thiab qis txoj hauj lwm ntawm lub condenser lens yuav txawv qhov luaj li cas ntawm lub aperture ntawm lub teeb nyeem ntawv thiab kev xaiv ntawm haum lim, uas yog tom qab ib tug tej lub sij hawm ntawm kev xyaum thiab kev.
Bio microscope Ib qho teeb meem tseem ceeb heev yog nyob rau hauv tus txheej txheem ntawm kev kuaj thiab sib cais ntawm cov hlwb, khov-qhuav thiab cob embedding (FD) tom qab khov ultra-booklets, khov-drying tom qab qhov zoo khiav ntawm txhua feem ntawm 65 lub ntsiab lus tseem ceeb yuav tsum yog. ua tib zoo ua tib zoo saib xyuas, thiab yuav tsum tsis txhob ua rau cov hlwb kom raug saib xyuas thiab tshuaj xyuas. Vim hais tias cov x-ray micro-chaw tsom xam tsis tsuas yog ntau cov kauj ruam, thiab tus nqi yog siab heev, yog tias tom qab lub sij hawm ntev thiab ntau cov kauj ruam ntawm kev ua, cov tshuaj ntsuam xyuas yog cov hlwb puas lossis cov hlwb tuag, kom kos cov lus xaus tsis raug. tu siab heev. Piv txwv li, cov cardiomyocytes cais los ntawm kev kho collagenase muaj ob lub morphologies, ib tug ntev pas nrig-puab thiab lwm yam yog puag ncig. Cov tom kawg yog cov hlwb tuag uas raug puas tsuaj thaum lub sijhawm sib cais ntawm tes.
Biomicroscopy Cov ntsiab lus thiab kev faib tawm ntawm electrolytes hauv ob hom cell no txawv heev. Na yog siab heev thiab K qis heev nyob rau hauv lub round cardiomyocytes, thiab cov concentration ntawm ca yog siab heev nyob rau hauv lub mitochondria. Tom qab kuaj xyuas nrog lwm txoj kev tshuaj ntsuam xyuas, nws tau pom tias qhov siab Na thiab qis K hauv cov hlwb puag ncig thiab lub siab ca hauv mitochondria yog qhov tshwm sim ntawm kev puas tsuaj rau cov cell membrane thaum lub sijhawm sib cais ntawm tes. Qhov txias fixation ntawm cov hlwb thiab cov ntaub so ntswg yog feem ntau ua los ntawm quench fixation tom qab preservation nyob rau hauv kua nitrogen. Quench fixation yog ib qho tseem ceeb rau kev ua tau zoo ntawm kev khaws cia. Nyob rau hauv lub cell los yog cov ntaub so ntswg tshiab yog nplua nuj nyob rau hauv dej, thaum quenching yog feem ntau cov hlwb los yog cov ntaub so ntswg thiab tus neeg ua hauj lwm txias (tshwj xeeb tshaj yog cov kua nitrogen ntshiab txias) ncaj qha kev sib cuag nrog rau qhov chaw ntawm thawj freeze-fixation, yog li tsim ib "plhaub", uas. tiv thaiv qhov chaw ntawm lub cell txias fixation. Raws li qhov tshwm sim, cov dej khov nab kuab feem ntau pom nyob hauv nruab nrab ntawm cov hlwb loj dua thaum kuaj x-ray microcellular. Txhawm rau tiv thaiv qhov no los ntawm qhov tshwm sim, cov tshuaj uas muaj cov ntsiab lus melting siab dua li cov kua nitrogen tab sis qis dua 806c qhuav yog siv los ua cov khoom ua kom txias. Muaj ntau yam khoom zoo li no, tab sis * yooj yim kom tau, pheej yig yog concentrated propane (qhov kub ntawm 42.120c, melting point ntawm 187.10c, molecular hnyav ntawm 44.1), qhov cua txias * ceev. Txawm li cas los xij, nws muaj qhov tsis zoo ntawm kev ua cov nplaim taws.
Lub tshuab kuaj kab mob lom neeg tuaj yeem muab tso rau ntawm lub txee tshwj xeeb cov leeg nqaij fiber ntau, kom cov nqaij fiber ntau contraction kom ncav cuag lub sijhawm yuav tsum tau kho, tam sim ntawd pib lub nozzle, kom cov kua propane rau cov leeg nqaij fiber ntau dav hlau, kom cov quenching. lub txias tsau. Tom qab ntawd cov nqaij fiber ntau raug muab tawm ua ke nrog lub khib thiab muab tso rau hauv cov kua nitrogen. Yog hais tias kho cov ntshav los yog cov hlwb raug rho tawm, thawj qhov qis qis centrifugation ua rau cov hlwb muaj zog, cov hlwb yuav raug xa mus rau qhov zoo thermal conductivity ntawm lub raj nyiaj, lub raj rau hauv cov kua propane carcass txias tsau. Hall lub chaw soj nstuam tsau nas pancreas, thawj ob daim hlau nrog kua helium (los yog kua nitrogen) ua ntej txias, nrog ib tug clamp clamped ob daim tooj liab yuav muab tso rau pem hauv ntej thiab tom qab txiav, kom tus phauj ntawm lub caj pas quenching txias fixation. Cov ntaub so ntswg lossis cov hlwb tuaj yeem khaws cia hauv cov kua nitrogen ntev ntev tom qab quenching thiab kho.
