Biological microscope ntawm qhov ntim ntawm cov ntaub so ntswg thaiv
Lub tshuab kuaj kab mob lom neeg nrog lub condenser tuaj yeem txav lub condenser nce thiab nqis kom nws qhov ci ntsa iab, thiab tseem tuaj yeem hloov qhov aperture ntawm lub teeb tsom teeb pom kev sib txawv kom ua tiav qhov ci ntsa iab ntawm 9b. Yog tias lub teeb nyob hauv lub hnub, lub condenser tuaj yeem tsa tau tsim nyog, thiab lub aperture ntawm lub suab nrov sib txawv tuaj yeem nthuav dav kom tsim nyog. Yog tias lub teeb muaj zog heev, lub condenser tuaj yeem txo qis kom tsim nyog, thiab qhov aperture ntawm kev sib tshuam tuaj yeem raug txo kom tsim nyog. Yog hais tias koj tseem xav tias dazzling nyob rau hauv cov ntaub ntawv no, koj muaj peev xwm xaiv ib tug tsim nyog lim thiab muab tso rau hauv lub bracket nyob rau hauv lub condenser. Tussah no tuaj yeem tau txais qhov ci ntsa iab uas txaus siab rau koj. Tau kawg, nws yog ib qho tsim nyog kom tau txais kev paub dhau los tom qab qee lub sijhawm ntawm kev coj ua hauv kev kho cov haujlwm sab saud thiab qis ntawm lub condenser los hloov qhov aperture loj ntawm qhov kev nyeem ntawv kho qhov muag thiab xaiv cov ntxaij lim dej uas tsim nyog.
Ib qho teeb meem tseem ceeb heev ntawm biomicroscope yog tias cov ntsiab lus ntawm 65 cov ntsiab lus hauv txhua qhov tom qab khov-qhuav thiab cov resin embedding (FD) thiab khov-drying yuav tsum tau ua tib zoo saib xyuas, thiaj li tsis ua rau lub hlwb puas yuav tsum tau soj ntsuam thiab soj ntsuam. Vim tias X-ray microanalysis tsis tsuas yog cuam tshuam nrog ntau cov kauj ruam, tab sis kuj raug nqi ntau, nws yog ib qho kev khuv xim heev los kos qhov tsis ncaj ncees lawm yog tias cov hlwb soj ntsuam yog cov hlwb puas lossis cov hlwb tuag tom qab kev kho mob ntev thiab ntau kauj ruam. Piv txwv li, myocardial hlwb sib cais los ntawm kev kho collagenase muaj ob daim ntawv, ib tug yog pas nrig ntev thiab lwm yam yog puag ncig. Cov tom kawg yog lub cell tuag uas puas thaum lub sijhawm sib cais ntawm tes.
Cov ntsiab lus thiab kev faib tawm ntawm electrolytes nyob rau hauv ob hom cell no txawv heev nyob rau hauv biological microscope. Na yog siab heev thiab K yog tsawg heev nyob rau hauv ib puag ncig myocardial hlwb, thiab ca concentration nyob rau hauv linear dendrites yog siab heev. Piv nrog rau lwm txoj kev tshuaj ntsuam xyuas, nws tau ua pov thawj tias qhov siab Na thiab qis K hauv cov hlwb ncig thiab qhov siab ca hauv mitochondria yog cov txiaj ntsig ntawm cell membrane puas thaum lub sijhawm sib cais ntawm tes. Txoj kev kho mob txias ntawm cov hlwb thiab cov ntaub so ntswg feem ntau yog kho lawv los ntawm quenching ua ntej, thiab tom qab ntawd khaws cia rau hauv cov kua nitrogen. Quenching fixation yog ib qho tseem ceeb heev rau cov nyhuv preservation. Cov cell nyob los yog cov ntaub so ntswg tshiab muaj dej ntau. Thaum quenching, nws feem ntau yog qhov chaw ntawm cov hlwb los yog cov ntaub so ntswg uas nyob rau hauv kev sib cuag ncaj qha nrog cryoprotectants (tshwj xeeb tshaj yog thaum quenching nrog kua nitrogen) yog khov thiab ruaj ua ntej, yog li tsim ib "plhaub", uas cuam tshuam lub khov thiab kho ntawm lub hauv paus. ib feem ntawm cov hlwb. Yog li ntawd, thaum ua X-ray microanalysis, nws feem ntau pom tias muaj cov dej khov hauv nruab nrab ntawm cov hlwb loj. Txhawm rau tiv thaiv qhov no los ntawm qhov tshwm sim, ib yam khoom uas muaj cov ntsiab lus melting siab tshaj li cov kua nitrogen tab sis qhov ziab kub qis dua 806c yog siv los ua tus neeg ua haujlwm txias. Muaj ntau yam xws li tshuaj, tab sis qhov yooj yim tshaj plaws uas muaj ib qho yog concentrated propane (boiling point -42.120c, melting point-187.10c, molecular hnyav-44.1), uas muaj cov cua txias ceev tshaj plaws. Tab sis nws qhov tsis zoo yog flammability.
Lub tshuab siv tshuaj lom neeg tuaj yeem muab cov nqaij fiber ntau tso rau ntawm ib qho tshwj xeeb, thiab thaum cov nqaij fiber ntau cog lus rau qee theem thiab yuav tsum tau kho, lub nozzle pib tam sim ntawd, kom cov kua propane yog txau rau ntawm cov leeg nqaij kom tua thiab kho. nws. Tom qab ntawd, cov nqaij fibers raug coj tawm ua ke nrog cov khib nyiab thiab muab tso rau hauv cov kua nitrogen. Yog tias cov qe ntshav los yog cov hlwb sib cais raug kho, thawj centrifuge ntawm qis ceev kom mloog zoo rau cov hlwb, hloov cov hlwb mus rau ib lub raj me me nrog cov thermal conductivity zoo, thiab muab cov raj me me tso rau hauv cov kua propane kom khov thiab kho. Thaum kho nas pancreas hauv Hall Laboratory, ob lub steel blocks tau precooled nrog kua helium (los yog kua nitrogen), thiab ob lub tooj liab blocks tau muab tso rau pem hauv ntej thiab tom qab txiav txiav nrog pliers, kom cov phauj tau quenched thiab tsau. Cov ntaub so ntswg lossis cov hlwb tuaj yeem khaws cia rau lub sijhawm ntev tom qab quenched thiab tsau thiab xa mus rau cov kua nitrogen.
