Kev siv tshuaj lom neeg - Laser Microscopy
1. Muaj feem xyuam rau cell biology, cell physiology, neurobiology thiab neurophysiology thiab yuav luag txhua yam kev tshawb fawb ntawm tes.
2. Kev soj ntsuam ntawm lub sijhawm tiag tiag uas tsis muaj kev puas tsuaj thiab kev soj ntsuam ntawm cov hlwb nyob, thiab kev tshawb fawb txog kev ua haujlwm morphological thiab kev ua haujlwm. Non-invasive, txhim khu kev qha thiab reproducible cell nrhiav tau; cov ntaub ntawv dluab tuaj yeem tso tawm hauv lub sijhawm lossis khaws cia rau lub sijhawm ntev.
3. Nruam tomography ntawm cov cell nyob thiab cov ntaub so ntswg los yog cov ntaub so ntswg ntawm tes, tuaj yeem tau txais ib lub xovtooj ntawm tes zoo lossis ib pawg ntawm cov hlwb lossis cov qauv ntawm cov ntaub so ntswg hauv zos hauv txhua theem (nrog rau ob sab thiab peb sab) (nrog rau cov qauv tshwj xeeb ntawm tes - xws li cytoskeleton, chromosomes, organelles thiab cell membrane system, cov qauv ntawm cov qauv sib sib zog nqus) thiab ua tiav peb-dimensional duab (xws li kev tsom xam ntawm cov kev hloov ntawm lub sij hawm), piv txwv li, plaub-dimensional duab, tab sis kuj los soj ntsuam cov hloov raws sijhawm. (xws li, txheeb xyuas cov kev hloov pauv hauv lub sijhawm, piv txwv li, cov duab plaub-dimensional, tab sis kuj hloov pauv cov fluorescence wavelengths, uas tso cai rau ntau cov duab ntau yam). Nrhiav qhov chaw spatial ntawm cov ntaub so ntswg thiab lwm yam khoom siv uas yuav tsum tau ua raws li kev soj ntsuam ntawm lub sijhawm tiag tiag, kev tsom xam thiab kev sau cia; ** Kev txheeb xyuas qhov zoo, ntau, sijhawm thiab qhov chaw faib khoom.
4. Fluorescent labeling probes labeled cellular biomaterials of living cells or sectioned specimens, membrane labelling, cellular tracers,
Cov tshuaj, tshuaj tiv thaiv, receptors lossis ligands, nucleic acids, thiab lwm yam. Kev soj ntsuam; ib txhij ntau yam tshuaj labeling thiab kev soj ntsuam ib txhij tuaj yeem ua rau tib tus qauv.
5. Fluorescent labeling ntawm intracellular ions, ib leeg lossis ntau daim ntawv lo, nrhiav kom pom ntawm intracellular xws li pH thiab sodium, potassium, calcium thiab magnesium.
6. Kev ntsuas ntawm cell membrane muaj peev xwm, nrhiav kom tau dawb radicals, thiab lwm yam;
7. Ua ** positioned fluorescence bleaching recovery thwmsim, ua ke nrog fluorescence poob nyob rau hauv fluorescence bleaching thwmsim, kev kawm ntawm intercellular kev sib txuas lus thiab lwm yam intracellular tshuaj (molecules, thiab lwm yam.) txav; nyob rau hauv lub sij hawm-scanning thwmsim thiab yees duab-bleaching (lub teeb quenching) thwmsim, yuav ua tau ib simultaneous tso zis thiab hloov dua siab tshiab ntawm cov ntaub ntawv thiab cov duab rau txhua channel. Ua fluorescence resonance zog hloov kev sim los kawm txog kev txav ntawm cov molecules thiab ions nyob rau hauv lub cell los ntawm kev hloov ntawm fluorescence wavelength thiab kev sib cuam tshuam.
8.Very (spatial positioning, quantification, fixed wavelength, fixed time), rhiab heev, ceev thiab tuaj yeem ua tiav tib lub sijhawm ntawm cov ntaub so ntswg ntawm tes ntawm ntau cov cim fluorescent (txawm tias emission wavelengths ze heev, xws li qhov sib txawv ntawm tsuas yog ib ob peb nm ntau fluorescence) ntawm ntau lub wavelengths ntawm cov duab sib cais thiab kev soj ntsuam thiab kev tsom xam, nrog rau ntau yam fluorescent markers co-localisation ntawm on-line ntsuas thiab tsom xam functions.
