Kev siv tshuaj lom neeg-laser confocal microscopy
1. Muaj feem xyuam rau yuav luag txhua qhov kev tshawb fawb ntawm tes xws li cell biology, cell physiology, neurobiology thiab neurophysiology.
2. Ua qhov tsis muaj kev puas tsuaj rau lub sijhawm tiag tiag thiab kev soj ntsuam ntawm cov hlwb nyob thiab ua cov kev tshawb fawb txog kev ua haujlwm morphological thiab kev ua haujlwm. Nws tsis ua rau muaj kev puas tsuaj rau lub xovtooj ntawm tes, muaj kev nyab xeeb, txhim khu kev qha thiab muaj kev rov ua tau zoo dua; cov ntaub ntawv dluab tuaj yeem tso tawm rau lub sijhawm lossis khaws cia rau lub sijhawm ntev.
3. Nruam tomography ntawm cov hlwb nyob thiab cov ntaub so ntswg los yog cov ntaub so ntswg ntawm tes tuaj yeem tau txais cov qauv qhia ntxaws (ob- thiab peb-dimensional) ntawm ib tus neeg lub hlwb lossis ib pawg ntawm cov hlwb lossis cov ntaub so ntswg hauv zos (xws li cov qauv tshwj xeeb ntawm tes - xws li cytoskeleton), chromosomes, organelles thiab cell membrane systems, cov qauv sib sib zog nqus ntawm cov qauv) thiab ua tiav cov duab peb sab (xws li kev txheeb xyuas cov kev hloov hauv lub sijhawm, uas yog, cov duab plaub-dimensional), thiab cov duab uas hloov nrog fluorescence wavelengths, uas tuaj yeem ua tiav ntau dua. cov duab seem. ). Nrhiav qhov chaw ntawm lub hlwb ntawm cov ntaub so ntswg thiab lwm yam khoom siv uas yuav tsum tau soj ntsuam, thiab ua qhov kev soj ntsuam ntawm lub sijhawm tiag tiag, kev tshuaj xyuas thiab kev sau cia; txheeb xyuas qhov zoo, qhov ntau, lub sijhawm thiab qhov chaw faib khoom.
4. Kev soj ntsuam ntawm cellular biomaterials, membrane markers, cell tracers, tshuaj, tshuaj, tshuaj tiv thaiv, receptors los yog ligands, nucleic acids, thiab lwm yam nyob rau hauv lub hlwb los yog hlais cov qauv uas muaj fluorescent labeling probes; simultaneous ntau yam khoom labeling tuaj yeem ua rau tib tus qauv, pom ib txhij.
5. Intracellular ion fluorescence labeling, ib daim ntawv lo lossis ntau daim ntawv lo, pom qhov sib piv thiab kev hloov pauv ntawm intracellular pH thiab ion concentrations xws li sodium, potassium, calcium, magnesium, thiab lwm yam;
6. Cell membrane muaj peev xwm ntsuas, kuaj dawb radical, thiab lwm yam;
7. Ua qhov kev sim tshuaj fluorescence bleaching hauv zos, ua ke nrog cov fluorescence poob thwmsim thaum lub sij hawm fluorescence bleaching, kawm intercellular kev sib txuas lus thiab kev txav ntawm lwm yam tshuaj intracellular (molecules, thiab lwm yam); nyob rau hauv lub sij hawm scanning thwmsim thiab photobleaching (photoquenching) thwmsim, koj muaj peev xwm Tib lub sij hawm, cov ntaub ntawv thiab dluab ntawm txhua channel yog tso zis thiab hloov dua siab tshiab ib txhij. Ua fluorescence resonance zog hloov kev sim los kawm txog kev txav thiab kev sib cuam tshuam ntawm intracellular molecules thiab ions los ntawm kev hloov pauv hauv fluorescence wavelength.
8. Nws yog qhov tseeb heev (spatial positioning, quantification, taag wavelength, fixed time), rhiab, ceev, thiab tuaj yeem ua tiav ntau yam fluorescent labeling ntawm cov ntaub so ntswg ntawm tes tib lub sijhawm (txawm tias cov emission wavelengths ze heev, xws li ntau yam fluorescence. nrog rau qhov sib txawv ntawm ob peb nm)), kev sib cais thiab kev soj ntsuam ntawm cov duab ntawm ntau lub wavelengths, nrog rau kev ntsuas hauv online thiab tsom xam ua haujlwm xws li kev sib koom ua ke ntawm ntau cov ntawv fluorescent
