Qhov zoo ntawm Confocal LaserMicroscopy
Hauv kev xyaum, cov lus qhia kev vibration ntawm lub sab sauv thiab qis polarizing lo ntsiab muag ntawm lub tshuab tsom iav polarizing yuav tsum yog orthogonal rau ib leeg, lossis nyob rau sab hnub tuaj-sab hnub poob lossis sab qaum teb-sab qab teb, thiab txhua tus yuav tsum nyob rau hauv txoj kab nrog tus ntoo khaub lig hlau ntawm lub qhov muag hauv cov lus qhia transverse thiab longitudinal. Qee zaum tsuas yog siv lub polariser qis dua rau kev soj ntsuam, kev co kev taw qhia ntawm lub polarizer qis yuav tsum tau txiav txim siab, yog li cov polariser yuav tsum tau kho thaum lub sijhawm ua haujlwm.
1, eyepiece cross filament nrhiav kom tau
Txhawm rau txheeb xyuas seb lub qhov muag hla qhov filament yog orthogonal, thiab seb nws puas zoo ib yam nrog cov kev taw qhia kev vibration ntawm sab sauv thiab sab polarizers. Nyob rau tib lub sijhawm, xaiv ib daim ntawm mica dub nrog kev daws teeb meem tag nrho, txav mus rau qhov nruab nrab ntawm lub qhov muag hla lub ntsej muag, tso qhov kev daws teeb meem hla mus rau ib qho ntawm cov ntoo khaub lig filaments, sau cov naj npawb ntawm cov nplai ntawm cov khoom thauj, Tom qab ntawd tig lub rooj tsiaj kom qhov kev daws teeb meem yog sib npaug mus rau lwm cov ntoo khaub lig filament, sau cov naj npawb ntawm cov nplais ntawm lub rooj nqa khoom, thiab qhov sib txawv ntawm ob qhov ntsuas yog 90 degree, uas qhia tau hais tias tus ntoo khaub lig filaments yog orthogonal.
2, Kev txiav txim siab thiab kho qhov kev co kev taw qhia ntawm qis polarizer
Siv cov mica dub los xyuas cov kev taw qhia kev co ntawm cov polarizer qis, vim tias cov mica dub yog cov pob tshab pob tshab dav dav, uas yog cov yam ntxwv ntawm ib lub teeb polarized. Ua ntej pom ib qho kev daws teeb meem thiab meej dub mica, txav mus rau qhov chaw ntawm lub qhov muag hla lub ntsej muag, tso lub polarizer sab saud, tig lub rooj nqa khoom rau ib lub lim tiam, thiab saib xyuas qhov kev hloov ntawm cov xim ntawm cov mica dub, vim tias cov mica dub. absorbs lub teeb vibration nyob rau hauv cov kev taw qhia ntawm kev daws teeb meem * muaj zog, yog li thaum cov xim dub mica ncav cuag * tob, cov kev taw qhia ntawm cov kev daws teeb meem slit yog kev co kev taw qhia ntawm lub polarizer qis.
3, Kho ntawm orthogonality ntawm sab sauv thiab sab polarizers
Tom qab cov kev taw qhia ntawm lub qis polarizer raug kho, tshem tawm cov ntawv thiab thawb nws mus rau sab sauv polarizer saib seb lub teb ntawm saib yog dub kiag li, piv txwv li seb nws yog nyob rau hauv ib tug extinction xeev. Yog tias nws yog tag nrho cov dub, nws txhais tau hais tias cov kev taw qhia kev co ntawm lub sab sauv thiab sab polarisers yog orthogonal rau ib leeg. Txwv tsis pub, lub sab sauv polariser yuav tsum raug kho, piv txwv li tig lub sab sauv polariser kom lub teb ntawm saib mus txog * tsaus. Thaum tig, nres ntsia hlau ntawm lub polarizer sab sauv yuav tsum tau xoob ua ntej, thiab tom qab ntawd nruj tom qab kho.
