Cov txiaj ntsig zoo thiab qhov tsis zoo ntawm Multiphoton laser scanning me me hauv biomedical imaging
Ntau lub photon laser scannope yog ib txoj kev sim raws li laser scannopy tshuab, uas muab ntau dua optical slicing lub peev xwm hauv peb {{} seem kev soj ntsuam. Qhov ntau photon fluorescence kevcai siv ntev wavelength ci lossis ze {} Kev daws teeb meem, nrog kev puas tsuaj tsawg rau cov quav dev. Yog li ntawd, nws yog qhov tsim nyog rau kev ua haujlwm ntawm cov hlwb nyob xws li lub hlwb slices, embryos, tag nrho cov kabmob, thiab txawm tias tag nrho lub cev.
Qhov zoo yog raws li nram no:
1. Zoo siab los ntawm cov xim liab lossis infrared, lub teeb tawg yog me me (qhov tawg ntawm cov khoom me me yog qhov ua tau zoo rau plaub lub wavelength).
2. Tsis muaj cov pinholes, muaj peev xwm sau ntau dua cov duab tawg ntawm cov ntoo khaub lig {}}}}} seem.
3. Pinnholes tsis tuaj yeem paub qhov txawv ntawm cov duab tawg tau emittefection los ntawm kev tiv thaiv lossis thaj chaw focal, thiab multiphotons muaj teeb liab zoo dua} {cov suab nrov piv rau hauv kev ua kom sib sib nqus.
4. Cov ulaviolet lossis pom lub teeb pom kev siv rau kev ua kom yooj yim tau yooj yim los ntawm cov nqaj ua ntej lub teeb pom kev ua ntej lub dav hlau xub ntiag, ua rau kom muaj qhov nyuaj rau kom muaj cov khaubncaws sab nraud pov thawj.
5. Nyob rau hauv cov nqe lus ntawm biological micrologopy kev soj ntsuam, thawj qhov kev txiav txim siab yog kom tsis txhob ua rau cov kab ncig ntawm cov kab ncig, thiab kom muaj kev suav, oxygen, thiab as-ham. Hauv kev saib xyuas lub teeb, ob qho tib si thermal thiab photon zog yuav tsum nyob hauv irradiation koob tshuaj thiab lub teeb hluav taws xob tsis ua rau lub hlwb ua kev puas tsuaj.
6. Multiphoton me me dua muaj ntau yam zoo. Nyob rau hauv cov nqe lus ntawm peb- qhov chaw nyob hauv, kev tiv thaiv qhov tob, kev tswj hwm, thiab lwm yam ntxiv tias laser tsom iav yav dhau los tsis muaj.
Multiphoton Compocal Faser Scannope lub tsom iav tau txuas ntxiv rau cov qauv kev tshawb fawb thiab kev hloov pauv ntawm cov khoom muaj peev xwm tom qab Multiphoton excitation, peb- Diaensional qhov siab yuav tsum tau ua tiav, cov khoom siv Laser Pauv Ntau Dua
