Muaj ntau hom fluorescence microscopy imaging cov txheej txheem
Txhawm rau kom ua tau raws li qhov xav tau sib txawv fluorescence imaging xav tau, ntxiv rau fluorescence microscopes, ntau yam fluorescence microscopy imaging daws tau tsim:
Cov duab qhia dav dav dav dav, xws li Leica THUNDER Imager, siv Leica lub thev naus laus zis thev naus laus zis kom tshem tawm cov teeb meem tsis sib haum xeeb ntawm lub dav hlau cuam tshuam thaum lub sijhawm ua duab, nthuav tawm cov duab meej, thiab muaj qhov zoo ntawm cov duab nrawm;
Confocal laser scanning microscope, siv cov pinholes kom tshem tawm cov kev cuam tshuam ntawm lub dav hlau uas tsis yog focal, paub txog kev kho qhov muag, thiab tau txais cov duab siab thiab cov duab peb sab;
Ultra-high-resolution microscopes thiab nano-microscopes uas tawg los ntawm qhov kev txwv tsis pub dhau, thiab tuaj yeem soj ntsuam cov qauv zoo me dua 200nm;
Multiphoton imaging system uas siv lub hauv paus ntsiab lus ntawm multiphoton excitation los ua cov duab sib sib zog nqus ntawm cov ntaub so ntswg thiab lub cev nyob;
Lub teeb-daim duab imaging tshuab nrog siab spatio-temporal daws teeb meem, ceev imaging ceev, siab daws teeb meem, tsis tshua muaj phototoxicity, tshwj xeeb tshaj yog haum rau kev loj hlob, nyob dynamic soj ntsuam thiab lwm yam kev tshawb fawb;
Fluorescence lifetime imaging (FLIM), uas tsis cuam tshuam los ntawm cov yam ntxwv xws li cov khoom siv fluorescent concentration, photobleaching, thiab excitation lub teeb siv zog, tuaj yeem ua ntau qhov tob thiab ntsuas kev ua haujlwm raug;
Fluorescence correlation spectroscopy (FCS) thiab fluorescence cross-correlation spectroscopy (FCCS), ntsuas tus naj npawb molecular thiab diffusion coefficient ntawm fluorescent molecules, thiaj li txheeb xyuas molecular concentration, molecular loj, viscosity, molecular motion, molecular binding / dissociation ntawm cov khoom. molecules, thiab lwm yam;
Tag nrho cov kev xav sab hauv fluorescence microscopy (TIRF), nrog rau qhov siab z-axis daws teeb meem, yog qhov tsim nyog rau kev kawm txog cov qauv molecular thiab dynamics ntawm lub cell membranes.
Fluorescence microscopy imaging technology yog dav siv thiab nplua nuj nyob rau hauv hom, thiab cov technologies tshiab tau tshwm sim tas li. Koj tuaj yeem xaiv cov cuab yeej tsim nyog los ua kom tiav koj li kev tshawb fawb.
